Stanniocalcin 2 Recombinant antibody, PBS Only (Capture)

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  WB analysis using 85910-1-RR (same clone as 85910-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85910-1-RR (STC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85910-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02212-1

  Sandwich ELISA standard curve of MP02212-1, Human Stanniocalcin 2 Recombinant Matched Antibody Pair - PBS only. 85910-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3119. 85910-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

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  Sample test of MP02212-1

  The mean Stanniocalcin 2 concentration was determined to be 113.80 ng/mL in T-47D cell extract based on a 2.3 mg/mL extract load.

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  Sample test of MP02212-1

  Serum of eight individual healthy human donors was measured. The human Stanniocalcin 2 concentration of detected samples was determined to be 4.42 ng/mL with a range of 0.30 - 12.67 ng/mL.

Stanniocalcin 2 Uni-rAb^TM Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 85910-1-PBS

Clone No.250328A9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

STC2, Stanniocalcin related protein, Stanniocalcin-2, Stanniocalcin-related protein, STC 2

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Product Information

85910-1-PBS targets Stanniocalcin 2 as part of a matched antibody pair:

MP02212-1: 85910-1-PBS capture and 85910-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Stanniocalcin 2 fusion protein Eg3119
• Full Name: stanniocalcin 2
• Calculated Molecular Weight: 33 kDa
• GenBank Accession Number: NM_003714.3
• Gene Symbol: Stanniocalcin 2
• Gene ID (NCBI): 8614
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O76061
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Stanniocalcin (STC) is a family of secreted glycoprotein hormones that originally discovered in the corpuscles of Stannius, an endocrine gland of fish. STC1 and STC2, two homologues of STC family, are reported to involve in calcium and phosphate homeostasis. It is expressed in a wide variety of tissues such as kidney, spleen, heart, and pancreas. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. STC2 overexpression could promote tumor cell proliferation, invasion and metastasis in prostate cancer, ovarian cancer or neuroblastoma. STC2 is also vital for cytoprotective properties when exposed to ER stress and hypoxia.