|Positive WB detected in||HeLa cells, HepG2 cells, Jurkat cells, K-562 cells|
|Positive IHC detected in||human ovary tumor tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:5000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11565-1-AP targets STIM1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||STIM1 fusion protein Ag2140|
|Full Name||stromal interaction molecule 1|
|Calculated molecular weight||685 aa, 77 kDa|
|Observed molecular weight||77-90 kDa|
|GenBank accession number||BC021300|
|Gene ID (NCBI)||6786|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER-plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.The MW of STIM1 is about 80-90 kDa which may be due to the phosphorylation or glycosylation forms. Catalog#11565-1-AP is rabbit polyclonal antibody raised against the N-terminal of human STIM1.
Cholesterylation of Smoothened is a calcium-accelerated autoreaction involving an intramolecular ester intermediate.
J Immunother Cancer
PD1 blockade enhances K+ channel activity, Ca2+ signaling, and migratory ability in cytotoxic T lymphocytes of patients with head and neck cancer.
Proc Natl Acad Sci U S A
STIM1-Ca2+ signaling modulates automaticity of the mouse sinoatrial node.
Int J Mol Sci
Expression and Localization of Thrombospondins, Plastin 3, and STIM1 in Different Cartilage Compartments of the Osteoarthritic Varus Knee.
Blockage of Orai1-Nucleolin interaction meditated calcium influx attenuates breast cancer cells growth
Aging (Albany NY)
Orai, STIM, and PMCA contribute to reduced calcium signal generation in CD8+ T cells of elderly mice.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Udesh (Verified Customer) (08-16-2023)
Worked well in WB at 1:1000
lieqi (Verified Customer) (12-19-2018)