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  • KD/KO Validated

STIM1 Polyclonal antibody

STIM1 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 11565-1-AP

Synonyms

D11S4896E, GOK, STIM1, stromal interaction molecule 1



Tested Applications

Positive WB detected inHepG2 cells, mouse liver tissue, HT-1080 cells, BxPC-3 cells, Raji cells, NIH/3T3 cells
Positive IP detected inRaji cells
Positive IHC detected inhuman ovary tumor tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11565-1-AP targets STIM1 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STIM1 fusion protein Ag2140
Full Name stromal interaction molecule 1
Calculated molecular weight 685 aa, 77 kDa
Observed molecular weight 80-90 kDa
GenBank accession numberBC021300
Gene symbol STIM1
Gene ID (NCBI) 6786
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER-plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.The MW of STIM1 is about 80-90 kDa which may be due to the phosphorylation or glycosylation forms. Catalog#11565-1-AP is rabbit polyclonal antibody raised against the N-terminal of human STIM1.

Protocols

Product Specific Protocols
WB protocol for STIM1 antibody 11565-1-APDownload protocol
IHC protocol for STIM1 antibody 11565-1-APDownload protocol
IF protocol for STIM1 antibody 11565-1-APDownload protocol
IP protocol for STIM1 antibody 11565-1-APDownload protocol
FC protocol for STIM1 antibody 11565-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EBioMedicine

FUN14 domain-containing 1 promotes breast cancer proliferation and migration by activating calcium-NFATC1-BMI1 axis.

Authors - Lianpin Wu
humanIF

Sci Rep

E-syt1 Re-arranges STIM1 Clusters to Stabilize Ring-shaped ER-PM Contact Sites and Accelerate Ca2+ Store Replenishment.

Authors - Fei Kang
mouseWB,IF

Proc Natl Acad Sci U S A

Remodeling of ER-plasma membrane contact sites but not STIM1 phosphorylation inhibits Ca2+ influx in mitosis.

Authors - Fang Yu
  • KO Validated
mouseIP

Cell Rep

PMID: 31801093

Authors - Benjamin D Stein
humanWB,IF

Cells

STIM Protein-NMDA2 Receptor Interaction Decreases NMDA-Dependent Calcium Levels in Cortical Neurons.

Authors - Joanna Gruszczynska-Biegala
  • KD Validated
human,mouseWB

Biochim Biophys Acta.

Presenilin-dependent expression of STIM proteins and dysregulation of capacitative Ca2+ entry in familial Alzheimer's disease.

Authors - Bojarski L

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

lieqi (Verified Customer) (12-19-2018)

  • Applications: Western Blot,
  • Cell Tissue Type: T84
STIM1 Antibody Western Blot, validation ( dilution) in T84 (Cat no:11565-1-AP)