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  • KD/KO Validated

STIM1 Polyclonal antibody

STIM1 Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)





Cat no : 11565-1-AP


D11S4896E, GOK, STIM1, stromal interaction molecule 1

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman ovary tumor tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Positive FC detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11565-1-AP targets STIM1 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STIM1 fusion protein Ag2140
Full Name stromal interaction molecule 1
Calculated Molecular Weight 685 aa, 77 kDa
Observed Molecular Weight 77-90 kDa
GenBank Accession NumberBC021300
Gene Symbol STIM1
Gene ID (NCBI) 6786
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER-plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.The MW of STIM1 is about 80-90 kDa which may be due to the phosphorylation or glycosylation forms. Catalog#11565-1-AP is rabbit polyclonal antibody raised against the N-terminal of human STIM1.


Product Specific Protocols
WB protocol for STIM1 antibody 11565-1-APDownload protocol
IHC protocol for STIM1 antibody 11565-1-APDownload protocol
IF protocol for STIM1 antibody 11565-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Res

Cholesterylation of Smoothened is a calcium-accelerated autoreaction involving an intramolecular ester intermediate.

Authors - Ao Hu
  • KO Validated

J Immunother Cancer

PD1 blockade enhances K+ channel activity, Ca2+ signaling, and migratory ability in cytotoxic T lymphocytes of patients with head and neck cancer.

Authors - Hannah S Newton
mouseWB, IF

Proc Natl Acad Sci U S A

STIM1-Ca2+ signaling modulates automaticity of the mouse sinoatrial node.

Authors - Hengtao Zhang

Cell Mol Life Sci

STIM2 regulates NMDA receptor endocytosis that is induced by short-term NMDA receptor overactivation in cortical neurons

Authors - Karolina Serwach
  • KD Validated
humanWB, IF

Int J Mol Sci

Expression and Localization of Thrombospondins, Plastin 3, and STIM1 in Different Cartilage Compartments of the Osteoarthritic Varus Knee.

Authors - Daniela Mählich


Blockage of Orai1-Nucleolin interaction meditated calcium influx attenuates breast cancer cells growth

Authors - Chunming Gu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Udesh (Verified Customer) (08-16-2023)

Worked well in WB at 1:1000

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Glioblastoma cells

lieqi (Verified Customer) (12-19-2018)

  • Applications: Western Blot,
  • Cell Tissue Type: T84
STIM1 Antibody Western Blot, validation ( dilution) in T84 (Cat no:11565-1-AP)