STIM1 Monoclonal antibody

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  WB analysis of K-562 using 66189-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66189-1-Ig (STIM1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66189-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66189-1-Ig (STIM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 66189-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66189-1-Ig (STIM1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66189-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66189-1-Ig (STIM1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66189-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66189-1-Ig (STIM1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human ovary tumor using 66189-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66189-1-Ig (STIM1 Antibody) at dilution of 1:200 (under 40x lens).

• Featured Product
• KD/KO Validated

STIM1 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 66189-1-Ig

Clone No.1D3A3

Publications(6)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

STRMK, stim-1, STIM 1, IMD10, GOK

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Tested Applications

• Positive WB detected in: K-562 cells, Jurkat cells
• Positive IHC detected in: human lung cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IF: See 2 publications below

Product Information

66189-1-Ig targets STIM1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17914

  Product name: Recombinant human STIM1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 2-350 aa of BC021300

  Sequence: DVCVRLALWLLWGLLLHQGQSLSHSHSEKATGTSSGANSEESTAAEFCRIDKPLCHSEDEKLSFEAVRNIHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVYNWTVDEVVQWLITYVELPQYEETFRKLQLSGHAMPRLAVTNTTMTGTVLKMTDRSHRQKLQLKALDTVLFGPPLLTRHNHLKDFMLVVSIVIGVGGCWFAYIQNRYSKEHMKKMMKDLEGLHRAEQSLHDLQERLHKAQEEHRTVEVEKVHLEKKLRDEINLAKQEAQRLKELREGTENERSRQKYAEEELEQVREALRKAEKELESHSSWYAPEALQKW

• Full Name: stromal interaction molecule 1
• Calculated Molecular Weight: 685 aa, 77 kDa
• Observed Molecular Weight: 77 kDa
• GenBank Accession Number: BC021300
• Gene Symbol: STIM1
• Gene ID (NCBI): 6786
• RRID: AB_2881584
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13586
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER-plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.The MW of STIM1 is about 80-90 kDa which may be due to the phosphorylation or glycosylation forms.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Int J Mol Sci; Neuroprotective Effect of Antiapoptotic URG7 Protein on Human Neuroblastoma Cell Line SH-SY5Y; Authors - Ilaria Nigro
rat	WB,IF	Biomed Pharmacother; Physical interaction of tropomyosin 3 and STIM1 regulates vascular smooth muscle contractility and contributes to hypertension.; Authors - Fangfang Xu ; KD Validated
mouse	WB	J Transl Med; Targeting unfolded protein response reverts ER stress and ER Ca2+ homeostasis in cardiomyocytes expressing the pathogenic variant of Lamin A/C R321X; Authors - Giusy Pietrafesa
human,mouse	WB,IF	Neurotoxicology; STIM1 Mediates Methamphetamine-Induced Neuronal Autophagy and Apoptosis; Authors - Qin Tian ; KD Validated
	WB	J Dairy Sci; Fatty acids promote migration of CD4+ T cells through calcium release-activated calcium modulator ORAI1 sensitive glycolysis in dairy cows; Authors - Bingbing Zhang
human	WB	Nat Cell Biol; Sphingosine-1-phosphate signalling activates E-Syt1 to facilitate HDL-derived cholesterol transport; Authors - Zizhen Xu ; KD Validated