Validation Data Gallery
|Positive WB detected in||K-562 cells, Jurkat cells|
|Positive IHC detected in||human lung cancer tissue, human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66189-1-Ig targets STIM1 in WB, IHC, IF, ELISA applications and shows reactivity with rat, human, mouse samples.
|Tested Reactivity||rat, human, mouse|
|Cited Reactivity||mouse, rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||STIM1 fusion protein Ag17914|
|Full Name||stromal interaction molecule 1|
|Calculated molecular weight||685 aa, 77 kDa|
|Observed molecular weight||77 kDa|
|GenBank accession number||BC021300|
|Gene ID (NCBI)||6786|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER-plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.The MW of STIM1 is about 80-90 kDa which may be due to the phosphorylation or glycosylation forms.
Physical interaction of tropomyosin 3 and STIM1 regulates vascular smooth muscle contractility and contributes to hypertension.
J Transl Med
Targeting unfolded protein response reverts ER stress and ER Ca2+ homeostasis in cardiomyocytes expressing the pathogenic variant of Lamin A/C R321X