STIM1 Monoclonal antibody

STIM1 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

rat, human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1D3A3

Cat no : 66189-1-Ig

Synonyms



Tested Applications

Positive WB detected inK-562 cells, Jurkat cells
Positive IHC detected inhuman lung cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66189-1-Ig targets STIM1 in WB, IHC, IF,ELISA applications and shows reactivity with rat, human, mouse samples.

Tested Reactivity rat, human, mouse
Cited Reactivity rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen STIM1 fusion protein Ag17914
Full Name stromal interaction molecule 1
Calculated molecular weight 685 aa, 77 kDa
Observed molecular weight 77 kDa
GenBank accession numberBC021300
Gene symbol STIM1
Gene ID (NCBI) 6786
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STIM1, also named as GOK, is an ER Ca2+ sensor. Upon Ca2+ store depletion, STIM1 clusters at ER–plasma membrane junctions where it interacts with and gates Ca2+-permeable Orai1 ion channels. STIM1 is implicated in tumor growth suppression and stromal-haematopoietic cell interactions. Defacts in STIM1 gene can cause immune dysfunction with impaired T-cell activation. STIM1 can inhibit L-type voltage-gated Ca(2+) channels in neurons. Temperature is an important regulator of STIM1 function.The MW of STIM1 is about 80-90 kDa which may be due to the phosphorylation or glycosylation forms.

Protocols

Product Specific Protocols
WB protocol for STIM1 antibody 66189-1-IgDownload protocol
IHC protocol for STIM1 antibody 66189-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IF

Biomed Pharmacother

Physical interaction of tropomyosin 3 and STIM1 regulates vascular smooth muscle contractility and contributes to hypertension.

Authors - Fangfang Xu
  • KD Validated