Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | mouse spleen tissue, human brain tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 11 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
Product Information
21192-1-AP targets STIM2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15530 Product name: Recombinant human STIM2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 525-674 aa of BC146661 Sequence: EKICGFQIAHNSGLPSLTSSLYSDHSWVVMPRVSIPPYPIAGGVDDLDEDTPPIVSQFPGTMAKPPGSLARSSSLCRSRRSIVPSSPQPQRAQLAPHAPHPSHPRHPHHPQHTPHSLPSPDPDILSVSSCPALYRNEEEEEAIYFSAEKQ Predict reactive species |
| Full Name | stromal interaction molecule 2 |
| Calculated Molecular Weight | 754 aa, 85 kDa |
| Observed Molecular Weight | 85-115 kDa |
| GenBank Accession Number | BC146661 |
| Gene Symbol | STIM2 |
| Gene ID (NCBI) | 57620 |
| RRID | AB_10734322 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9P246 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
STIM2, also named as KIAA1482, functions as a highly sensitive Ca2+ sensor in the endoplasmic reticulum which activates both store-operated and store-independent Ca2+-influx. Regulates basal cytosolic and endoplasmic reticulum Ca2+ concentrations. Upon mild variations of the endoplasmic reticulum Ca2+ concentration, STIM2 translocates from the endoplasmic reticulum to the plasma membrane where it probably activates the Ca2+ release-activated Ca2+ (CRAC) channels ORAI1, ORAI2 and ORAI3. STIM2 may inhibit STIM1-mediated Ca2+ influx. STIM2 has 2 isoforms 105kd and 115kd with Differential levels of phosphorylation.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for STIM2 antibody 21192-1-AP | Download protocol |
| IP protocol for STIM2 antibody 21192-1-AP | Download protocol |
| WB protocol for STIM2 antibody 21192-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Res Cholesterylation of Smoothened is a calcium-accelerated autoreaction involving an intramolecular ester intermediate.
| ||
Cell Mol Life Sci STIM2 regulates NMDA receptor endocytosis that is induced by short-term NMDA receptor overactivation in cortical neurons
| ||
Oncotarget The calcium channel proteins ORAI3 and STIM1 mediate TGF-β induced Snai1 expression.
| ||
Neurobiol Dis Inhibiting store-operated calcium entry attenuates white matter secondary degeneration following SCI. | ||
Biochem J STIM1 negatively regulates Ca2+ release from the sarcoplasmic reticulum in skeletal myotubes. | ||
Cell Signal RGS10 physically and functionally interacts with STIM2 and requires store-operated calcium entry to regulate proinflammatory gene expression in microglia.
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