STK16 Monoclonal antibody

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  WB analysis using 60949-3-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60949-3-Ig (STK16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 60949-3-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 60949-3-Ig (STK16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

STK16 Monoclonal Antibody for WB, ELISA

Cat No. 60949-3-Ig

Clone No.2D6C6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Synonyms

EC:2.7.10.2, EC:2.7.11.1, hPSK, KRCT, MPSK

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Tested Applications

• Positive WB detected in: Ramos cells, LNCaP cells, Ramos, THP-1, Karpas-299, MJ cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60949-3-Ig targets STK16 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30523

  Product name: Recombinant human STK16 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 2-207 aa of BC002618

  Sequence: GHALCVCSRGTVIIDNKRYLFIQKLGEGGFSYVDLVEGLHDGHFYALKRILCHEQQDREEAQREADMHRLFNHPNILRLVAYCLRERGAKHEAWLLLPFFKRGTLWNEIERLKDKGNFLTEDQILWLLLGICRGLEAIHAKGYAHRDLKPTNILLGDEGQPVLMDLGSMNQACIHVEGSRQALTLQDWAAQRCTISYRAPELFSVQ

• Full Name: serine/threonine kinase 16
• Calculated Molecular Weight: 39.4 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC002618
• Gene Symbol: STK16
• Gene ID (NCBI): 8576
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O75716
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STK16 is a Golgi-associated serine/threonine kinase that regulates membrane trafficking, Golgi organization, and cell-cycle progression. Through constitutive kinase activity and lipid-mediated membrane association, STK16 links vesicular transport with cellular proliferation and polarity. Its dysregulation in cancer highlights STK16 as a functional node connecting intracellular logistics and growth control.