Tested Applications
| Positive WB detected in | BxPC-3 cells, human skeletal muscle tissue, mouse skeletal muscle tissue, NIH/3T3 cells |
| Positive IHC detected in | human heart tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
Product Information
14433-1-AP targets STK17A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5811 Product name: Recombinant human STK17A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-326 aa of BC047696 Sequence: MIPLEKPGSGGSSPGATSGSGRAGRGLSGPCRPPPPPQARGLLTEIRAVVRTEPFQDGYSLCPGRELGRGKFAVVRKCIKKDSGKEFAAKFMRKRRKGQDCRMEIIHEIAVLELAQDNPWVINLHEVYETASEMILVLEYAAGGEIFDQCVADREEAFKEKDVQRLMRQILEGVHFLHTRDVVHLDLKPQNILLTSESPLGDIKIVDFGLSRILKNSEELREIMGTPEYVAPEILSYDPISMATDMWSIGVLTYVMLTGISPFLGNDKQETFLNISQMNLSYSEEEFDVLSESAVDFIRTLLVKKPEDRATAEECLKHPWLTQSSI Predict reactive species |
| Full Name | serine/threonine kinase 17a |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 47-53 kDa |
| GenBank Accession Number | BC047696 |
| Gene Symbol | STK17A |
| Gene ID (NCBI) | 9263 |
| RRID | AB_10858629 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UEE5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
STK17A (serine/threonine kinase 17A), also known as DRAK1 (death-associated protein kinase-related apoptosis-inducing kinase 1), is a serine/threonine protein kinase belonging to the DAPK family. STK17A is primarily localized in the nucleus and functions as a regulator of apoptosis, cell proliferation, and the cellular response to DNA damage. Its expression is induced by stress signals, including p53 activation, where it contributes to cell cycle arrest and programmed cell death. In addition to its role in apoptosis, STK17A has been implicated in the regulation of cytoskeletal organization, cell migration, and transcriptional responses to cellular stress.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for STK17A antibody 14433-1-AP | Download protocol |
| WB protocol for STK17A antibody 14433-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Med Rep Prognostic and predictive roles of microRNA‑411 and its target STK17A in evaluating radiotherapy efficacy and their effects on cell migration and invasion via the p53 signaling pathway in cervical cancer.
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