STK17A Polyclonal antibody

• Featured Product
• KD/KO Validated

STK17A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14433-1-AP

Synonyms

DRAK1, serine/threonine kinase 17a, STK17A

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Validation Data Gallery

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  WB analysis of BxPC-3 using 14433-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14433-1-AP (STK17A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14433-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14433-1-AP (STK17A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 14433-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14433-1-AP (STK17A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 14433-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14433-1-AP (STK17A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 14433-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14433-1-AP (STK17A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 14433-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14433-1-AP (STK17A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 14433-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14433-1-AP (STK17A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 14433-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14433-1-AP (STK17A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 14433-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14433-1-AP (STK17A antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: BxPC-3 cells, human skeletal muscle tissue, mouse skeletal muscle tissue, NIH/3T3 cells
• Positive IHC detected in: human heart tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

14433-1-AP targets STK17A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STK17A fusion protein Ag5811
• Full Name: serine/threonine kinase 17a
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47-53 kDa
• GenBank Accession Number: BC047696
• Gene Symbol: STK17A
• Gene ID (NCBI): 9263
• RRID: AB_10858629
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Med Rep; Prognostic and predictive roles of microRNA‑411 and its target STK17A in evaluating radiotherapy efficacy and their effects on cell migration and invasion via the p53 signaling pathway in cervical cancer.; Authors - Wei Wei ; KD Validated