STK32C Polyclonal antibody

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  WB analysis using 11434-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11434-1-AP (STK32C antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 11434-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using STK32C antibody (11434-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

STK32C Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 11434-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

EC:2.7.11.1, PKE, RP11 140A10.1, serine/threonine kinase 32C, Serine/threonine-protein kinase 32C

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11434-1-AP targets STK32C in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STK32C fusion protein Ag1960
• Full Name: serine/threonine kinase 32C
• Calculated Molecular Weight: 486 aa, 55 kDa
• Observed Molecular Weight: 43-55 kDa
• GenBank Accession Number: BC015792
• Gene Symbol: STK32C
• Gene ID (NCBI): 282974
• RRID: AB_2196626
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86UX6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STK32C (Serine/Threonine Kinase 32C) is a member of the AGC family of serine/threonine protein kinases. STK32C is overexpressed in various cancers, including bladder cancer and prostate cancer. In bladder cancer, high expression of STK32C is associated with poor clinical outcomes and short relapse-free survival (PMID: 30359551).