|Positive WB detected in||human brain tissue, rat brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11311-1-AP targets STMN3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||STMN3 fusion protein Ag1853|
|Full Name||stathmin-like 3|
|Calculated molecular weight||21 kDa|
|Observed molecular weight||21-25 kDa|
|GenBank accession number||BC009381|
|Gene ID (NCBI)||50861|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Stathmin family phosphoproteins participate in the control of microtubule dynamics and have been proposed to be involved in the control of neuronal differentiation. Neuron specific Stathmin-3 (STMN3) is a novel STAT3 (signal transducer and activator of transcription 3) interacting protein. STAT3 is a key contributor to cancer cell migration and invasion. STMN3 exhibits microtubule-destabilizing activity, which is antagonized by STAT3. SCG10 is a widely studied and recognized neuronal differentiation marker, STMN3 is a SCG10-like protein (SCLIP) and is present from the earliest stages of hippocampal neuron differentiation in culture at vesicle-like structures following dynamic microtubules. RNAi mediated inhibition of this gene resulted in increased axonal branching.
Coordinated expression of stathmin family members by far upstream sequence element-binding protein-1 increases motility in non-small cell lung cancer.
RUNX1 is a driver of renal cell carcinoma correlating with clinical outcome.
HAT cofactor TRRAP modulates microtubule dynamics via SP1 signaling to prevent neurodegeneration.
Mol Cancer Ther
J Biol Chem
The Stathmin-2 membrane targeting domain is required for axon protection and regulated degradation by DLK signaling
BMC Cell Biol
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