STMN3 Polyclonal antibody

• Featured Product
• KD/KO Validated

STMN3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 11311-1-AP

Synonyms

SCG10 like protein, SCLIP, Stathmin 3, stathmin like 3, STMN3

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Validation Data Gallery

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  WB analysis of human brain using 11311-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11311-1-AP (STMN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 11311-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11311-1-AP (STMN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11311-1-AP

  IP Result of anti-STMN3 (IP:11311-1-AP, 3ug; Detection:11311-1-AP 1:2000) with mouse brain tissue lysate 8000ug.

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  IHC staining of human colon cancer using 11311-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11311-1-AP (STMN3 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: human brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

11311-1-AP targets STMN3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STMN3 fusion protein Ag1853
• Full Name: stathmin-like 3
• Calculated molecular weight: 21 kDa
• Observed molecular weight: 21-25 kDa
• GenBank accession number: BC009381
• Gene symbol: STMN3
• Gene ID (NCBI): 50861
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Stathmin family phosphoproteins participate in the control of microtubule dynamics and have been proposed to be involved in the control of neuronal differentiation. Neuron specific Stathmin-3 (STMN3) is a novel STAT3 (signal transducer and activator of transcription 3) interacting protein. STAT3 is a key contributor to cancer cell migration and invasion. STMN3 exhibits microtubule-destabilizing activity, which is antagonized by STAT3. SCG10 is a widely studied and recognized neuronal differentiation marker, STMN3 is a SCG10-like protein (SCLIP) and is present from the earliest stages of hippocampal neuron differentiation in culture at vesicle-like structures following dynamic microtubules. RNAi mediated inhibition of this gene resulted in increased axonal branching.

Publications

Species	Application	Title
human	WB, IHC	Cancer Res; Coordinated expression of stathmin family members by far upstream sequence element-binding protein-1 increases motility in non-small cell lung cancer.; Authors - Stephan Singer
human	WB	Cancer Res; RUNX1 is a driver of renal cell carcinoma correlating with clinical outcome.; Authors - Nicholas Rooney
mouse	WB,IF	Elife; HAT cofactor TRRAP modulates microtubule dynamics via SP1 signaling to prevent neurodegeneration.; Authors - Alicia Tapias
human	WB	Mol Cancer Ther; PMID: 23543364; Authors - Xie Xuemei X ; KD Validated
	WB	BMC Cell Biol; CiteAb: a searchable antibody database that ranks antibodies by the number of times they have been cited.; Authors - Matthew A Helsby
mouse	WB	Cell Rep; Loss of Stathmin-2, a hallmark of TDP-43-associated ALS, causes motor neuropathy.; Authors - Kelsey L Krus