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  • KD/KO Validated

STMN3 Polyclonal antibody

STMN3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11311-1-AP

Synonyms

SCG10 like protein, SCLIP, Stathmin 3, stathmin like 3, STMN3



Tested Applications

Positive WB detected inhuman brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11311-1-AP targets STMN3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STMN3 fusion protein Ag1853
Full Name stathmin-like 3
Calculated molecular weight 21 kDa
Observed molecular weight 21-25 kDa
GenBank accession numberBC009381
Gene symbol STMN3
Gene ID (NCBI) 50861
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Stathmin family phosphoproteins participate in the control of microtubule dynamics and have been proposed to be involved in the control of neuronal differentiation. Neuron specific Stathmin-3 (STMN3) is a novel STAT3 (signal transducer and activator of transcription 3) interacting protein. STAT3 is a key contributor to cancer cell migration and invasion. STMN3 exhibits microtubule-destabilizing activity, which is antagonized by STAT3. SCG10 is a widely studied and recognized neuronal differentiation marker, STMN3 is a SCG10-like protein (SCLIP) and is present from the earliest stages of hippocampal neuron differentiation in culture at vesicle-like structures following dynamic microtubules. RNAi mediated inhibition of this gene resulted in increased axonal branching.

Protocols

Product Specific Protocols
WB protocol for STMN3 antibody 11311-1-APDownload protocol
IHC protocol for STMN3 antibody 11311-1-APDownload protocol
IP protocol for STMN3 antibody 11311-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

BMC Cell Biol

CiteAb: a searchable antibody database that ranks antibodies by the number of times they have been cited.

Authors - Matthew A Helsby
humanWB

Mol Cancer Ther

PMID: 23543364

Authors - Xie Xuemei X
  • KD Validated
mouseWB,IF

Elife

HAT cofactor TRRAP modulates microtubule dynamics via SP1 signaling to prevent neurodegeneration.

Authors - Alicia Tapias
humanWB

Cancer Res

RUNX1 is a driver of renal cell carcinoma correlating with clinical outcome.

Authors - Nicholas Rooney
humanWB, IHC

Cancer Res

Coordinated expression of stathmin family members by far upstream sequence element-binding protein-1 increases motility in non-small cell lung cancer.

Authors - Stephan Singer