STT3A Monoclonal antibody

STT3A Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1E2B12

Cat no : 66581-1-Ig

Synonyms

B5, Integral membrane protein 1, ITM1, STT3 A, STT3A, TMC, Transmembrane protein TMC



Tested Applications

Positive WB detected inTHP-1 cells, K-562 cells, HL-60 cells, HEK-293 cells, RAW 264.7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66581-1-Ig targets STT3A in WB, IHC,ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen STT3A fusion protein Ag27072
Full Name STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)
Calculated molecular weight 705 aa, 81 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC020965
Gene symbol STT3A
Gene ID (NCBI) 3703
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STT3A, also named as Integral membrane protein 1, belongs to the STT3 family. STT3A is expressed at high levels in placenta, liver, muscle and pancreas, and at very low levels in brain, lung and kidney. STT3A is a catalytic subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. STT3A is present in the majority of OST complexes and mediates cotranslational N-glycosylation of most sites on target proteins, while STT3B-containing complexes are required for efficient post-translational glycosylation and mediate glycosylation of sites that have been skipped by STT3A. There are two isoforms of STT3A with molecular weight of 81 and 70 kDa. 66581-1-Ig antibody detects a protein around 65-70 kDa in SDS-PAGE which is similar to papers published. (PMID: 32005703, 25135935)

Protocols

Product Specific Protocols
WB protocol for STT3A antibody 66581-1-IgDownload protocol
IHC protocol for STT3A antibody 66581-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols