|Positive WB detected in||THP-1 cells, K-562 cells, HL-60 cells, HEK-293 cells, RAW 264.7 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66581-1-Ig targets STT3A in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.
|Tested Reactivity||Human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||STT3A fusion protein Ag27072|
|Full Name||STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)|
|Calculated molecular weight||705 aa, 81 kDa|
|Observed molecular weight||65 kDa|
|GenBank accession number||BC020965|
|Gene ID (NCBI)||3703|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
STT3A, also named as Integral membrane protein 1, belongs to the STT3 family. STT3A is expressed at high levels in placenta, liver, muscle and pancreas, and at very low levels in brain, lung and kidney. STT3A is a catalytic subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. STT3A is present in the majority of OST complexes and mediates cotranslational N-glycosylation of most sites on target proteins, while STT3B-containing complexes are required for efficient post-translational glycosylation and mediate glycosylation of sites that have been skipped by STT3A. There are two isoforms of STT3A with molecular weight of 81 and 70 kDa. 66581-1-Ig antibody detects a protein around 65-70 kDa in SDS-PAGE which is similar to papers published. (PMID: 32005703, 25135935)
Transl Lung Cancer Res
Targeting STT3A produces an anti-tumor effect in lung adenocarcinoma by blocking the MAPK and PI3K/AKT signaling pathway.
Analysis of Tumor Glycosylation Characteristics and Implications for Immune Checkpoint Inhibitor's Efficacy for Breast Cancer.