Syntaxin 6 Polyclonal antibody

• 

  WB analysis of A549 using 10841-1-AP

  WB result of Syntaxin 6 antibody (10841-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Syntaxin 6 transfected A549 cells.

• 

  WB analysis using 10841-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10841-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10841-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10841-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10841-1-AP (Syntaxin 6 antibody at dilution of 1:2000 incubated at 4 degree celsius over night.

• 

  IP experiment of mouse brain using 10841-1-AP

  IP result of anti-Syntaxin 6 (IP:10841-1-AP, 3ug; Detection:10841-1-AP 1:1000) with mouse brain tissue lysate 7000ug.

• 

  IHC staining of mouse brain using 10841-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10841-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10841-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human prostate cancer using 10841-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10841-1-AP (Syntaxin 6 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 10841-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10841-1-AP (Syntaxin 6 antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of PC-12 using 10841-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using 66477-1-Ig (TGN46 antibody) at dilution of 1:100 and 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:50, further stained with CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66477-1-Ig, and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 10841-1-AP.

• 

  IF Staining of PC-12 using 10841-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using Syntaxin 6 antibody (10841-1-AP) at dilution of 1:700 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of PC-12 using 10841-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using Syntaxin 6 antibody (10841-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of murine BM-DC using 10841-1-AP

  IF result of anti-STX6 (10841-1-AP) in Murine BM-DC by Dr. LOREDANA SAVEANU.

• 

  IF Staining of murine Spleen-DC using 10841-1-AP

  IF result of anti-STX6 (10841-1-AP) in Murine Spleen-DC by Dr. LOREDANA SAVEANU.

• Featured Product
• KD/KO Validated

Syntaxin 6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10841-1-AP

Publications(23)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Syntaxin-6, STX6

---

Tested Applications

• Positive WB detected in: Neuro-2a cells, human brain tissue, mouse brain tissue, rat brain tissue, A549 cells, PC-12 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-12 cells, murine BM-DC, murine Spleen-DC

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:350-1:1400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 14 publications below
• FC: See 1 publications below

Product Information

10841-1-AP targets Syntaxin 6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1284

  Product name: Recombinant human STX6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-255 aa of BC009944

  Sequence: MSMEDPFFVVKGEVQKAVNTAQGLFQRWTELLQDPSTATREEIDWTTNELRNNLRSIEWDLEDLDETISIVEANPRKFNLDATELSIRKAFITSTRQVVRDMKDQMSTSSVQALAERKNRQALLGDSGSQNWSTGTTDKYGRLDRELQRANSHFIEEQQAQQQLIVEQQDEQLELVSGSIGVLKNMSQRIGGELEEQAVMLEDFSHELESTQSRLDNVMKKLAKVSHMTSDRRQWCAIAILFAVLLVVLILFLVL

• Full Name: syntaxin 6
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC009944
• Gene Symbol: Syntaxin 6
• Gene ID (NCBI): 10228
• RRID: AB_2196506
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43752
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syntaxin-6 (STX6) is a SNARE protein located in the trans-Golgi network (TGN) and endosomes. It associates with a variety of SNARE proteins and is involved in intracellular vesicle trafficking. STX6 is required for sorting proteins from endosomes toward either the TGN or lysosomes.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell; The WDR11 complex is a receptor for acidic-cluster-containing cargo proteins; Authors - Huaqing Deng
mouse	IF	Science; IRAP identifies an endosomal compartment required for MHC class I cross-presentation.; Authors - Loredana Saveanu
mouse	IF	Nat Immunol; IRAP(+) endosomes restrict TLR9 activation and signaling.; Authors - Joel Babdor
human	WB	Cell Rep Med; Compromised mitochondrial quality control triggers lipin1-related rhabdomyolysis.; Authors - Yamina Hamel
	IF,FC	Nat Commun; IRAP-dependent endosomal T cell receptor signalling is essential for T cell responses.; Authors - Irini Evnouchidou
mouse	IF	Cell Rep; Innate Immune Signals Induce Anterograde Endosome Transport Promoting MHC Class I Cross-Presentation.; Authors - Mirjana Weimershaus