Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, LNCaP cells, HepG2 cells, L02 cells, SMMC-7721 cells, human colostrum tissue, pig liver tissue |
| Positive IHC detected in | human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66477-1-Ig targets TGN46 in WB, IHC, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4470 Product name: Recombinant human TGOLN2,TGN46 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 25-384 aa of BC028219 Sequence: SVKQEEAGVRPSAGNVSTHPSLSQRPGGSTKSHPEPQTPKDSPSKSSAEAQTPEDTPNKSGAEAKTQKDSSNKSGAEAKTQKGSTSKSGSEAQTTKDSTSKSHPELQTPKDSTGKSGAEAQTPEDSPNRSGAEAKTQKDSPSKSGSEAQTTKDVPNKSGADGQTPKDGSSKSGAEDQTPKDVPNKSGAEKQTPKDGSNKSGAEEQGPIDGPSKSGAEEQTSKDSPNKVVPEQPSRKDHSKPISNPSDNKELPKADTNQLADKGKLSPHAFKTESGEETDLISPPQEEVKSSEPTEDVEPKEAEDDDTGPEEGSPPKEEKEKMSGSASSENREGTLSDSTGSEKDDLYPNGSGNGSAESSH Predict reactive species |
| Full Name | trans-golgi network protein 2 |
| Calculated Molecular Weight | 447 aa, 47 kDa |
| Observed Molecular Weight | 90 kDa |
| GenBank Accession Number | BC028219 |
| Gene Symbol | TGN46 |
| Gene ID (NCBI) | 10618 |
| RRID | AB_2881843 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O43493 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TGN46 (TGOLN2), the human homologue of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TGN46 antibody 66477-1-Ig | Download protocol |
| WB protocol for TGN46 antibody 66477-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TGN46 Monoclonal antibody (Cat# 66477-1-Ig) has 14 citations spanning journals including Nature Communications, Science Advances, Autophagy, Molecular Metabolism, Journal of Lipid Research, iScience, FEBS Journal, and Journal of Virology. Research fields covered include endosomal trafficking and autophagy, STING signaling, NLRP3 inflammasome biology, LDL transcytosis, Golgi network vesicle transport, metabolic health, viral infection mechanisms, and cerebral organoid quality control.
| Species | Application | Title |
|---|---|---|
Autophagy WNT2B impairs endosomal trafficking via WASHC5 to inhibit autophagy: a novel non-secretory WNT pathway. | ||
Nat Commun Negative feedback regulation of STING signaling by TAX1BP1-directed Golgiphagy. | ||
Sci Adv Isocyanic acid-mediated NLRP3 carbamoylation reduces NLRP3-NEK7 interaction and limits inflammasome activation | ||
Int J Biol Sci Annexin A5 Derived from Cell-free Fat Extract Attenuates Osteoarthritis via Macrophage Regulation | ||
Arterioscler Thromb Vasc Biol IL-1β Induces LDL Transcytosis by a Novel Pathway Involving LDLR and Rab27a | ||
Cell Mol Life Sci ELAPOR1 mediated vesicle traffic is required for acrosome biogenesis and male fertility in mice. |
Reviews
What customers say
The single review for this product is a 5-star rating from an Immunofluorescence application. The reviewer praised the antibody for producing clear images that were easily detected under the microscope, calling it the best option for IF work. Overall, the feedback highlights strong signal clarity and reliable performance in immunofluorescence experiments.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Karla (Verified Customer) (12-20-2022) | Proteintech has the best for IMF. Made my images clear and was picked up easily on the microscope.
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