SUDS3 Polyclonal antibody

SUDS3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25845-1-AP

Synonyms

SAP45, SDS3, SUDS3

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Validation Data Gallery

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  WB analysis of Raji using 25845-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 25845-1-AP (SUDS3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 25845-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 25845-1-AP (SUDS3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Raji cells, HL-60 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25845-1-AP is SUDS3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SUDS3 fusion protein Ag22961
• Full Name: suppressor of defective silencing 3 homolog (S. cerevisiae)
• Calculated Molecular Weight: 328 aa, 38 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC112000
• Gene Symbol: SUDS3
• Gene ID (NCBI): 64426
• RRID: AB_2880265
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SUDS3 (Sin3 histone deacetylase corepressor complex component SDS3), also known as SAP45 andSDS3, is a component of the Sin3 histone deacetylase corepressor complex. In this complex SDS3 interacts with SIN3A to regulate gene expression via chromatin modification. SUDS3 represses transcription and augments histone deacetylase activity of HDAC1. It may have a potential role in tumor suppressor pathways through regulation of apoptosis.