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  • KD/KO Validated

SURF1 Polyclonal antibody

SURF1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15379-1-AP

Synonyms

SURF 1, SURF1, surfeit 1, Surfeit locus protein 1



Tested Applications

Positive WB detected inhuman heart tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

15379-1-AP targets SURF1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SURF1 fusion protein Ag3959
Full Name surfeit 1
Calculated molecular weight 33 kDa
Observed molecular weight 30-31 kDa
GenBank accession numberBC028314
Gene symbol SURF1
Gene ID (NCBI) 6834
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SURF1 antibody 15379-1-APDownload protocol
IHC protocol for SURF1 antibody 15379-1-APDownload protocol
IF protocol for SURF1 antibody 15379-1-APDownload protocol
IP protocol for SURF1 antibody 15379-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Gene

SURF1 mutations in Chinese patients with Leigh syndrome: Novel mutations, mutation spectrum, and the functional consequences.

Authors - Yuanyuan Li
  • KO Validated