SURF1 Polyclonal antibody

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  WB analysis using 15379-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15379-1-AP (SURF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15379-1-AP

  IP result of anti-SURF1 (IP:15379-1-AP, 4ug; Detection:15379-1-AP 1:2000) with HeLa cells lysate 1575 ug.

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  IHC staining of human colon using 15379-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15379-1-AP (SURF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 15379-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15379-1-AP (SURF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15379-1-AP

  Immunofluorescent analysis of HeLa cells using 15379-1-AP (SURF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

SURF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15379-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Surfeit locus protein 1, surfeit 1, SURF-1, SURF 1

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, rat heart tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below

Product Information

15379-1-AP targets SURF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SURF1 fusion protein Ag3959
• Full Name: surfeit 1
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 30-31 kDa
• GenBank Accession Number: BC028314
• Gene Symbol: SURF1
• Gene ID (NCBI): 6834
• RRID: AB_2239968
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15526
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Gene; SURF1 mutations in Chinese patients with Leigh syndrome: Novel mutations, mutation spectrum, and the functional consequences.; Authors - Yuanyuan Li ; KO Validated
human	WB	Cell Rep; Mitochondrial respiration restricts Listeria monocytogenes infection by slowing down host cell receptor recycling.; Authors - Anna Spier ; KD Validated