Serpin E1/PAI-1 Recombinant monoclonal antibody

• 

  WB analysis of mouse placenta using 83980-3-RR

  mouse placenta tissue was subjected to SDS PAGE followed by western blot with 83980-3-RR (Serpin E1/PAI-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 83980-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83980-3-RR against Mouse Serpin E1/PAI-1 were performed. The affinity constant is 0.293 nM.

Serpin E1/PAI-1 Recombinant Antibody for WB, ELISA

Cat No. 83980-3-RR

Clone No.241130G6

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

PAI-1, Serpine1, Serpin E1, Plasminogen activator inhibitor 1, PAI1

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Tested Applications

• Positive WB detected in: mouse placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83980-3-RR targets Serpin E1/PAI-1 in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Fusion Protein

• Full Name: serine (or cysteine) peptidase inhibitor, clade E, member 1
• Calculated Molecular Weight: 45kDa
• Observed Molecular Weight: 45-50 kDa
• Gene Symbol: Serpin E1/PAI-1
• Gene ID (NCBI): 18787
• RRID: AB_3671554
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P22777
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERPINE1, also named as Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tPA and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia.