Anti-Human Siglec-9 Rabbit Recombinant Antibody

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  FC experiment of human peripheral blood leukocytes using 98264-6-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.125 ug Anti-Human Siglec-9 Rabbit RecAb (98264-6-RR, Clone: 241799C10) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using 98264-6-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.125 ug Anti-Human Siglec-9 Rabbit RecAb (98264-6-RR, Clone: 241799C10) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98264-6-RR

  Biolayer interferometry (BLl) kinetic assays of 98264-6-RR against Human Siglec-9 were performed. The affinity constant is 0.682 nM.

Siglec-9 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98264-6-RR

Clone No.241799C10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

SIGLEC9, Siglec 9, Sialic acid-binding Ig-like lectin 9, Protein FOAP 9, CD329

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.13 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98264-6-RR targets Siglec-9 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: sialic acid binding Ig-like lectin 9
• Calculated Molecular Weight: 50 kDa
• GenBank Accession Number: NM_014441.3
• Gene Symbol: Siglec-9
• Gene ID (NCBI): 27180
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y336-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Sialic acid binding Ig-like lectin 9 (Siglec-9), also known as CD329, is a member of the Siglec family of glycan-recognition proteins. Siglec-9 is a type-I transmembrane protein consisting of an N-terminal extracellular region that contains an N-terminal V-set domain and two C2-set domains, a transmembrane region, and an intracellular domain with an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an ITIM-like motif (PMID: 10801862). It is expressed quite broadly among human blood leukocytes, including monocytes, neutrophils, B cells, NK cells, and minor subsets of T cells (PMID: 32322597). Siglec-9 functions as an inhibitory immune checkpoint and can be targeted to enhance therapeutic antitumor immunity (PMID: 34155121; 37460871).