TAB1 Polyclonal antibody

• Featured Product
• KD/KO Validated

TAB1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 27566-1-AP

Synonyms

3' Tab1, MAP3K7IP1, TAB1, TAK1 binding protein 1

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Validation Data Gallery

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  WB analysis using 27566-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27566-1-AP (TAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 27566-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 27566-1-AP (TAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 27566-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27566-1-AP (TAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27566-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27566-1-AP (TAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 27566-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 27566-1-AP (TAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 27566-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 27566-1-AP (TAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 27566-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27566-1-AP (TAB1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 27566-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27566-1-AP (TAB1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 27566-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27566-1-AP (TAB1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 27566-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27566-1-AP (TAB1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, A375 cells, K-562 cells, NIH/3T3 cells
• Positive IHC detected in: human liver tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

27566-1-AP targets TAB1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TAB1 fusion protein Ag25576
• Full Name: mitogen-activated protein kinase kinase kinase 7 interacting protein 1
• Calculated molecular weight: 55 kDa
• Observed molecular weight: 55 kDa
• GenBank accession number: BC050554
• Gene symbol: MAP3K7IP1
• Gene ID (NCBI): 10454
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Theranostics; Virus-induced p38 MAPK activation facilitates viral infection.; Authors - Yuting Cheng ; KO Validated