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- KD/KO Validated
transgelin/SM22 Polyclonal antibody
transgelin/SM22 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat and More (1)
WB, IHC, IF, FC, ELISA
Cat no : 10493-1-AP
Validation Data Gallery
|Positive WB detected in||mouse colon tissue, Caco-2 cells, human colon tissue|
|Positive IHC detected in||human heart tissue, mouse heart tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse heart tissue|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 2 publications below|
|WB||See 68 publications below|
|IHC||See 13 publications below|
|IF||See 33 publications below|
|FC||See 1 publications below|
10493-1-AP targets transgelin/SM22 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, canine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||transgelin/SM22 fusion protein Ag0764|
|Calculated molecular weight||22 kDa|
|Observed molecular weight||19-22 kDa|
|GenBank accession number||BC004927|
|Gene ID (NCBI)||6876|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The transgelin family is a group of proteins that belong to 22kd actin-related calponin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody was generated against full length transgelin 1 protein. It can cross-react with other two transgelins based on the sequence similarity.
|Product Specific Protocols|
|WB protocol for transgelin/SM22 antibody 10493-1-AP||Download protocol|
|IHC protocol for transgelin/SM22 antibody 10493-1-AP||Download protocol|
|IF protocol for transgelin/SM22 antibody 10493-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Ying (Verified Customer) (08-19-2019)
very good antibody