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transgelin/SM22 Polyclonal antibody

transgelin/SM22 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10493-1-AP

Synonyms

SM22, SM22 alpha, SM22a, SM22alpha, SMCC, TAGLN, TAGLN1, transgelin, transgelin/SM22



Tested Applications

Positive WB detected inmouse colon tissue, Caco-2 cells, human colon tissue, mouse stomach tissue, rat colon tissue
Positive IHC detected inmouse heart tissue, human colon cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10493-1-AP targets transgelin/SM22 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, canine, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen transgelin/SM22 fusion protein Ag0764
Full Name transgelin
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 19-22 kDa
GenBank Accession NumberBC004927
Gene Symbol TAGLN
Gene ID (NCBI) 6876
RRIDAB_2199363
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The transgelin family is a group of proteins that belong to 22kd actin-related calponin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody was generated against full length transgelin 1 protein. It can cross-react with other two transgelins based on the sequence similarity.

Protocols

Product Specific Protocols
WB protocol for transgelin/SM22 antibody 10493-1-APDownload protocol
IHC protocol for transgelin/SM22 antibody 10493-1-APDownload protocol
IF protocol for transgelin/SM22 antibody 10493-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell

Mapping the Fine-Scale Organization and Plasticity of the Brain Vasculature.

Authors - Christoph Kirst
mouseWB,IF

Cell Host Microbe

Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation

Authors - Zhenyu Tian
mouseIF

Acta Pharm Sin B

The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway.

Authors - Weimin Yu
mouseIHC

J Exp Med

Dependence of proliferative vascular smooth muscle cells on CD98hc (4F2hc, SLC3A2).

Authors - Fogelstrand Per P
mouseIF

Nat Commun

Hyperlipidemia-induced cholesterol crystal production by endothelial cells promotes atherogenesis.

Authors - Yvonne Baumer
mouseIF

Circ Res

Peptide-mediated disruption of calmodulin-cyclin E interactions inhibits proliferation of vascular smooth muscle cells and neointima formation.

Authors - Hui Sonya S

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ying (Verified Customer) (08-19-2019)

very good antibody

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1000
  • Cell Tissue Type: primary mouse cardiomyocytes