|Positive WB detected in||mouse colon tissue, Caco-2 cells, human colon tissue|
|Positive IHC detected in||human heart tissue, mouse heart tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse heart tissue|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10493-1-AP targets transgelin/SM22 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||transgelin/SM22 fusion protein Ag0764|
|Calculated molecular weight||22 kDa|
|Observed molecular weight||19-22 kDa|
|GenBank accession number||BC004927|
|Gene ID (NCBI)||6876|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The transgelin family is a group of proteins that belong to 22kd actin-related calponin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody was generated against full length transgelin 1 protein. It can cross-react with other two transgelins based on the sequence similarity.
Antioxid Redox Signal
Peroxisome proliferator-activated receptor-γ coactivator-1α inhibits vascular calcification through sirtuin 3‒mediated reduction of mitochondrial oxidative stress.
Eur J Pharmacol
Death-associated protein kinase 3 deficiency alleviates vascular calcification via AMPK-mediated inhibition of endoplasmic reticulum stress.
Poly(ADP-ribose) polymerase 1 accelerates vascular calcification by upregulating Runx2.
Transactivation domain of Krüppel-like factor 15 negatively regulates angiotensin II-induced adventitial inflammation and fibrosis.
CTRP13 attenuates vascular calcification by regulating Runx2.
Biochem Biophys Res Commun
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Ying (Verified Customer) (08-19-2019)
very good antibody