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transgelin/SM22 Polyclonal antibody
transgelin/SM22 Polyclonal Antibody for IF, IHC, WB, ELISA
Cat no : 10493-1-AP
Validation Data Gallery
|Positive WB detected in
|mouse colon tissue, Caco-2 cells, human colon tissue, mouse stomach tissue, rat colon tissue
|Positive IHC detected in
|mouse heart tissue, human colon cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|mouse heart tissue
|Western Blot (WB)
|WB : 1:5000-1:50000
|IHC : 1:50-1:500
|IF : 1:200-1:800
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
10493-1-AP targets transgelin/SM22 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat, canine
|Host / Isotype
|Rabbit / IgG
|transgelin/SM22 fusion protein Ag0764
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
The transgelin family is a group of proteins that belong to 22kd actin-related calponin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody was generated against full length transgelin 1 protein. It can cross-react with other two transgelins based on the sequence similarity.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Ying (Verified Customer) (08-19-2019)
very good antibody