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CoraLite®594-conjugated transgelin/SM22 Polyclonal antibody
transgelin/SM22 Polyclonal Antibody for IF
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF
Conjugate
CoraLite®594 Fluorescent Dye
Cat no : CL594-10493
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | mouse heart tissue |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
CL594-10493 targets transgelin/SM22 in IF applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | transgelin/SM22 fusion protein Ag0764 |
Full Name | transgelin |
Calculated molecular weight | 22 kDa |
Observed molecular weight | 19-22 kDa |
GenBank accession number | BC004927 |
Gene symbol | TAGLN |
Gene ID (NCBI) | 6876 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The transgelin family is a group of proteins that belong to 22kd actin-related calponin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody was generated against full length transgelin 1 protein. It can cross-react with other two transgelins based on the sequence similarity.
Protocols
Product Specific Protocols | |
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IF protocol for CL594 transgelin/SM22 antibody CL594-10493 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |