TAPBPL Monoclonal antibody, PBS Only

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  WB analysis using 68577-1-Ig (same clone as 68577-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68577-1-Ig (TAPBPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68577-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68577-1-Ig (same clone as 68577-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68577-1-Ig (TAPBPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68577-1-PBS in a different storage buffer formulation.

• 

  FC experiment of A431 using 68577-1-Ig (same clone as 68577-1-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.4 ug TAPBPL Monoclonal antibody (68577-1-Ig, Clone: 1D12C2) and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002) (red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 68577-1-PBS in a different storage buffer formulation.

TAPBPL Monoclonal Antibody for WB, FC (Intra), Indirect ELISA

Cat No. 68577-1-PBS

Clone No.1D12C2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

Tapasin like, TAPASIN R, Tapasin-like, TAPASIN-R, Tapasin-related protein

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Product Information

68577-1-PBS targets TAPBPL in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14371

  Product name: Recombinant human TAPBPL protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 24-400 aa of BC015017

  Sequence: HPAEGQWRAVDVVLDCFLVKDGAHRGALASSEDRARASLVLKQVPVLDDGSLEDFTDFQGGTLAQDDPPIIFEASVDLVQIPQAEALLHADCSGKEVTCEISRYFLQMTETTVKTAAWFMANVQVSGGGPSISLVMKTPRVAKNEVLWHPTLNLPLSPQGTVRTAVEFQVMTQTQSLSFLLGSSASLDCGFSMAPGLDLISVEWRLQHKGRGQLVYSWTAGQGQAVRKGATLEPAQLGMARDASLTLPGLTIQDEGTYICQITTSLYRAQQIIQLNIQASPKVRLSLANEALLPTLICDIAGYYPLDVVVTWTREELGGSPAQVSGASFSSLRQSVAGTYSISSSLTAEPGSAGATYTCQVTHISLEEPLGASTQVV

• Full Name: TAP binding protein-like
• Calculated Molecular Weight: 468 aa, 50 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC015017
• Gene Symbol: TAPBPL
• Gene ID (NCBI): 55080
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9BX59
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TAPBPL, also named as TAPBP-R and TAPASIN-R, is a member of the variable-constant Ig superfamily that links MHC class I molecules to the transporter associated with antigen processing in the endoplasmic reticulum.