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TDP-43 (C-terminal) Polyclonal antibody
TDP-43 (C-terminal) Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
WB, IP, IHC, IF, CoIP, ChIP, ELISA
Conjugate
Unconjugated
212
Cat no : 12892-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, mouse brain tissue, HeLa cells, C6 cells |
Positive IP detected in | HeLa cells, mouse brain tissue |
Positive IHC detected in | mouse brain tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells, Neuro-2a cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:5000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:2000-1:8000 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 11 publications below |
WB | See 129 publications below |
IHC | See 41 publications below |
IF | See 81 publications below |
IP | See 10 publications below |
CoIP | See 2 publications below |
ChIP | See 2 publications below |
Product Information
12892-1-AP targets TDP-43 (C-terminal) in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, chicken, monkey, zebrafish, Drosophila |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | fusion protein |
Full Name | TAR DNA binding protein |
Calculated molecular weight | 43 kDa |
Observed molecular weight | 43-45 kDa, 35 kDa |
GenBank accession number | BC001487 |
Gene symbol | TARDBP |
Gene ID (NCBI) | 23435 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 12892-1-AP is a rabbit polyclonal antibody raised against the C-terminal amino acids of human TDP-43. This antibody recognizes the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phosphoprotein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (17023659,19823856,21666678,22193176)
Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (29581274)
Protocols
Product Specific Protocols | |
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WB protocol for TDP-43 (C-terminal) antibody 12892-1-AP | Download protocol |
IHC protocol for TDP-43 (C-terminal) antibody 12892-1-AP | Download protocol |
IF protocol for TDP-43 (C-terminal) antibody 12892-1-AP | Download protocol |
IP protocol for TDP-43 (C-terminal) antibody 12892-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Science HSP70 chaperones RNA-free TDP-43 into anisotropic intranuclear liquid spherical shells.
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Nature Therapeutic reduction of ataxin-2 extends lifespan and reduces pathology in TDP-43 mice. | ||
Nat Cell Biol Heat-shock chaperone HSPB1 regulates cytoplasmic TDP-43 phase separation and liquid-to-gel transition |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Parijat (Verified Customer) (09-09-2023) | Good antibody
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FH Xin (Verified Customer) (01-23-2022) | Good performance in WB (around 45 kD)
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FH Azita (Verified Customer) (06-17-2021) | Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by TDP-43 (C-terminal) Polyclonal antibody at dilution of 1:500 showed strong labelling.
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FH Jacob (Verified Customer) (03-25-2021) | Good for IF staining. It worked for cells fixation with 4% PFA.
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FH David (Verified Customer) (01-13-2020) | Good for both immunofluorescence and immunoblotting. Single band in control cells in the latter, but can detect stress induced C-terminal fragments.
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FH Alinda (Verified Customer) (02-28-2019) | Good antibody
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FH Noemi (Verified Customer) (01-24-2019) | really good antibody, it detects also the cleavage product of TDP43 in WB.
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