Filter: AllWBIHCIF/ICC

WB analysis using 24667-1-AP (same clone as 24667-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 24667-1-AP (TBC1D4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.
WB analysis using 24667-1-AP (same clone as 24667-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 24667-1-AP (TBC1D4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 24667-1-AP (same clone as 24667-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24667-1-AP (TBC1D4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 24667-1-AP (same clone as 24667-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon slide using 24667-1-AP (TBC1D4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 24667-1-AP (same clone as 24667-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon slide using 24667-1-AP (TBC1D4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 24667-1-AP (same clone as 24667-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24667-1-AP (TBC1D4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 24667-1-AP (same clone as 24667-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart slide using 24667-1-AP (TBC1D4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 24667-1-AP (same clone as 24667-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart slide using 24667-1-AP (TBC1D4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 24667-1-AP (same clone as 24667-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TBC1D4 antibody (24667-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24667-1-PBS in a different storage buffer formulation.

TBC1D4 Polyclonal antibody, PBS Only

TBC1D4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 24667-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

24667-1-PBS targets TBC1D4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag20327 Product name: Recombinant human TBC1D4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-200 aa of BC151239 Sequence: MEPPSCIQDEPFPHPLEPEPGVSAQPGPGKPSDKRFRLWYVGGSCLDHRTTLPMLPWLMAEIRRRSQKPEAGGCGAPAAREVILVLSAPFLRCVPAPGAGASGGTSPSATQPNPAVFIFEHKAQHISRFIHNSHDLTYFAYLIKAQPDDPESQMACHVFRATDPSQVPDVISSIRQLSKAAMKEDAKPSKDNEDAFYNSQ Predict reactive species
Full Name	TBC1 domain family, member 4
Calculated Molecular Weight	1299 aa, 147 kDa
Observed Molecular Weight	160 kDa
GenBank Accession Number	BC151239
Gene Symbol	TBC1D4
Gene ID (NCBI)	9882
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O60343
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

TBC1D4, also named as AS160, KIAA0603, may act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake. The antibody recognizes all the isoform of TBC1D4.