TBC1D7 Polyclonal antibody

• 

  WB analysis of human placenta using 20769-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 20769-1-AP (TBC1D7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20769-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20769-1-AP (TBC1D7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Neuro-2a using 20769-1-AP

  IP result of anti-TBC1D7 (IP:20769-1-AP, 4ug; Detection:20769-1-AP 1:500) with Neuro-2a cells lysate 1440 ug.

• 

  IP experiment of HeLa using 20769-1-AP

  IP result of anti-TBC1D7 (IP:20769-1-AP, 4ug; Detection:20769-1-AP 1:500) with HeLa cells lysate 1200 ug.

TBC1D7 Polyclonal Antibody for WB, IP, ELISA

Cat No. 20769-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Synonyms

PIG51, TBC1 domain family member 7, TBC1 domain family, member 7, TBC7

---

Tested Applications

• Positive WB detected in: Ramos cells, human placenta tissue, U266 cells, Neuro-2a cells
• Positive IP detected in: Neuro-2a cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20769-1-AP targets TBC1D7 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14756

  Product name: Recombinant human TBC1D7 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-293 aa of BC007054

  Sequence: MTEDSQRNFRSVYYEKVGFRGVEEKKSLEILLKDDRLDTEKLCTFSQRFPLPSMYRALVWKVLLGILPPHHESHAKVMMYRKEQYLDVLHALKVVRFVSDATPQAEVYLRMYQLESGKLPRSPSFPLEPDDEVFLAIAKAMEEMVEDSVDCYWITRRFVNQLNTKYRDSLPQLPKAFEQYLNLEDGRLLTHLRMCSAAPKLPYDLWFKRCFAGCLPESSLQRVWDKVVSGSCKILVFVAVEILLTFKIKVMALNSAEKITKFLENIPQDSSDAIVSKAIDLWHKHCGTPVHSS

• Full Name: TBC1 domain family, member 7
• Calculated Molecular Weight: 293 aa, 34 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC007054
• Gene Symbol: TBC1D7
• Gene ID (NCBI): 51256
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9P0N9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBC1D7 is a member of the TBC-domain containing protein family. The encoded protein functions as a subunit of the tuberous sclerosis TSC1-TSC2 complex which plays a role in the regulation of cellular growth and differentiation. Mutations in this gene have been associated with autosomal recessive megalencephaly.