TBC1D9B Polyclonal antibody

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  WB analysis using 20177-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20177-1-AP (TBC1D9B antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 20177-1-AP

  IP result of anti-TBC1D9B (IP:20177-1-AP, 4ug; Detection:20177-1-AP 1:1000) with Jurkat cells lysate 1920 ug.

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  IP experiment of HEK-293 using 20177-1-AP

  IP result of anti-TBC1D9B (IP:20177-1-AP, 4ug; Detection:20177-1-AP 1:1000) with HEK-293 cells lysate 1800 ug.

TBC1D9B Polyclonal Antibody for WB, IP, ELISA

Cat No. 20177-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

TBC1 domain family member 9B

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells
• Positive IP detected in: Jurkat cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20177-1-AP targets TBC1D9B in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14019

  Product name: Recombinant human TBC1D9B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1069-1250 aa of BC000701

  Sequence: ARTGRKPRDCATEEDEPPAPELHQDAARELQPPAAGDPQAKAGGDTHLGTAPQESQVVVEGGSGEGQGSPSQLLSDDETKDDMSMSSYSVVSTGSLQCEDLADDTVLVGGEACSPTARIGGTVDTDWCISFEQILASILTESVLVNFFEKRVDIGLKIKDQKKVERQFSTASDHEQPGVSG

• Full Name: TBC1 domain family, member 9B (with GRAM domain)
• Calculated Molecular Weight: 1250 aa, 140 kDa
• Observed Molecular Weight: 141-150 kDa
• GenBank Accession Number: BC000701
• Gene Symbol: TBC1D9B
• Gene ID (NCBI): 23061
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q66K14
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBC1 domain family member 9B (TBC1D9B) a Rab11A GTPase-activating protein (Rab11A GAP), as a LMTK1 binding protein, and TBC1D9B inactivates Rab11A under the control of LMTK1A (PMID: 31628178). TBC1D9B is associated with endocytic vesicles, including those likely to contain active Rab11a (PMID: 25232007). TBC1D9B could also interact with other mammalian ATG8 homologues. TBC1D9B could be co-localized with LC3B on the autophagosome membranes (PMID: 30202024).