"TBP Antibodies" Comparison
View side-by-side comparison of TBP antibodies from other vendors to find the one that best suits your research needs.
|Positive WB detected in
|LNCaP cells, HSC-T6 cells, HEK-293 cells, pig liver tissue, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, Neuro-2a cells
|Positive IP detected in
|Positive IHC detected in
|human liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|A431 cells, HeLa cells
|Positive FC detected in
|Western Blot (WB)
|WB : 1:20000-1:100000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:325-1:1300
|IF : 1:200-1:800
|Flow Cytometry (FC)
|FC : 1:10-1:100
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66166-1-Ig targets TBP in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
|human, mouse, rat, pig
|human, mouse, rat, pig
|Host / Isotype
|Mouse / IgG2a
|TBP fusion protein Ag12383
|TATA box binding protein
|Calculated molecular weight
|338 aa, 38 kDa
|Observed molecular weight
|mouse/rat 33-36 kDa and human 37-43kDa
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody detects human TBP (~40 kDa) and mouse/rat Tbp (~35 kDa).
|Product Specific Protocols
|WB protocol for TBP antibody 66166-1-Ig
|IHC protocol for TBP antibody 66166-1-Ig
|IF protocol for TBP antibody 66166-1-Ig
|IP protocol for TBP antibody 66166-1-Ig
|FC protocol for TBP antibody 66166-1-Ig
|Click here to view our Standard Protocols
Ligand-induced native G-quadruplex stabilization impairs transcription initiation.
Loss of adenomatous polyposis coli function renders intestinal epithelial cells resistant to the cytokine IL-22.
Inhibition of cell proliferation by Tas of foamy viruses through cell cycle arrest or apoptosis underlines the different mechanisms of virus-host interactions.
PPARα regulates the expression of human arylacetamide deacetylase involved in drug hydrolysis and lipid metabolism.
TFEB Promotes Prostate Cancer Progression via Regulating ABCA2-Dependent Lysosomal Biogenesis.
Mol Cancer Res
Identification of Endogenous Adenomatous Polyposis Coli Interaction Partners and β-Catenin-Independent Targets by Proteomics.