TBP Monoclonal antibody
TBP Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat, pig
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
2H3B2
Cat no : 66166-1-Ig
Synonyms
"TBP Antibodies" Comparison
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Tested Applications
Positive WB detected in | HEK-293 cells, pig liver tissue |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human liver cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | A431 cells, HeLa cells |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:325-1:1300 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 16 publications below |
Product Information
66166-1-Ig targets TBP in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, mouse, rat, pig |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | TBP fusion protein Ag12383 |
Full Name | TATA box binding protein |
Calculated molecular weight | 338 aa, 38 kDa |
Observed molecular weight | mouse/rat 33-36 kDa and human 37-43kDa |
GenBank accession number | BC110341 |
Gene symbol | TBP |
Gene ID (NCBI) | 6908 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody detects human TBP (~40 kDa) and mouse/rat Tbp (~35 kDa).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for TBP antibody 66166-1-Ig | Download protocol |
IHC protocol for TBP antibody 66166-1-Ig | Download protocol |
IF protocol for TBP antibody 66166-1-Ig | Download protocol |
IP protocol for TBP antibody 66166-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Int J Oncol Adaptive EGF expression sensitizes pancreatic cancer cells to ionizing radiation through activation of the cyclin D1/P53/PARP pathway. | ||
PLoS Biol Loss of adenomatous polyposis coli function renders intestinal epithelial cells resistant to the cytokine IL-22. | ||
Cell Rep Shift in MSL1 alternative polyadenylation in response to DNA damage protects cancer cells from chemotherapeutic agent-induced apoptosis. | ||
Cell Rep RPAP2 regulates a transcription initiation checkpoint by inhibiting assembly of pre-initiation complex. |