TBP Monoclonal antibody

TBP Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2H3B2

Cat no : 66166-1-Ig

Synonyms

GTF2D, GTF2D1, SCA17, TATA binding factor, TATA box binding protein, TATA box factor, TATA sequence binding protein, TBP, TF2D, TFIID


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  • Western Blot (WB) analysis of various lysates using TBP Monoclonal antibody (66166-1-Ig)

    WB analysis using 66166-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66166-1-Ig (TBP antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of various lysates using TBP Monoclonal antibody (66166-1-Ig)

    WB analysis using 66166-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66166-1-Ig (TBP antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TBP Monoclonal antibody (66166-1-Ig)

    WB analysis of HEK-293 using 66166-1-Ig

    Western blot analysis of TBP in various cell lines using Proteintech antibody 66166-1-Ig at a dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using TBP Monoclonal antibody (66166-1-Ig)

    WB analysis of pig liver using 66166-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 66166-1-Ig (TBP Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using TBP Monoclonal antibody (66166-1-Ig)

    IP experiment of HEK-293 using 66166-1-Ig

    IP result of anti-TBP (IP:66166-1-Ig, 3ug; Detection:66166-1-Ig 1:800) with HEK-293 cells lysate 1800ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TBP Monoclonal antibody (66166-1-Ig)

    IHC staining of human liver cancer using 66166-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66166-1-Ig (TBP Antibody) at dilution of 1:650 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TBP Monoclonal antibody (66166-1-Ig)

    IHC staining of human breast cancer using 66166-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66166-1-Ig (TBP Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TBP Monoclonal antibody (66166-1-Ig)

    IHC staining of human breast cancer using 66166-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66166-1-Ig (TBP Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TBP Monoclonal antibody (66166-1-Ig)

    IHC staining of human breast cancer using 66166-1-Ig

    After antigen unmasking with Tris-EDTA buffer (pH 9.0) for 15 mins and 2M HCL treatment for 30mins, Immunohistochemistry of paraffin-embedded human breast cancer tissue slideusing 66166-1-Ig (TBP Antibody) at dilution of 1:400 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TBP Monoclonal antibody (66166-1-Ig)

    IHC staining of human breast cancer using 66166-1-Ig

    I After antigen unmasking with Tris-EDTA buffer (pH 9.0) for 15 mins and 2M HCL treatment for 30mins, Immunohistochemistry of paraffin-embedded human breast cancer tissue slideusing 66166-1-Ig (TBP Antibody) at dilution of 1:400 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TBP Monoclonal antibody (66166-1-Ig)

    IHC staining of human liver cancer using 66166-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66166-1-Ig (TBP Antibody) at dilution of 1:650 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TBP Monoclonal antibody (66166-1-Ig)

    IF Staining of HeLa using 66166-1-Ig

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66166-1-Ig(TBP antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using TBP Monoclonal antibody (66166-1-Ig)

    IF Staining of A431 using 66166-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TBP antibody (66166-1-Ig, Clone: 2H3B2 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

"TBP Antibodies" Comparison

View side-by-side comparison of TBP antibodies from other vendors to find the one that best suits your research needs.

Tested Applications

Positive WB detected inLNCaP cells, HSC-T6 cells, HEK-293 cells, pig liver tissue, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, Neuro-2a cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:325-1:1300
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66166-1-Ig targets TBP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TBP fusion protein Ag12383
Full Name TATA box binding protein
Calculated Molecular Weight 338 aa, 38 kDa
Observed Molecular Weight mouse/rat 33-36 kDa and human 37-43kDa
GenBank Accession NumberBC110341
Gene Symbol TBP
Gene ID (NCBI) 6908
RRIDAB_2881562
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody detects human TBP (~40 kDa) and mouse/rat Tbp (~35 kDa).

Protocols

Product Specific Protocols
WB protocol for TBP antibody 66166-1-IgDownload protocol
IHC protocol for TBP antibody 66166-1-IgDownload protocol
IF protocol for TBP antibody 66166-1-IgDownload protocol
IP protocol for TBP antibody 66166-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Genome Res

Ligand-induced native G-quadruplex stabilization impairs transcription initiation.

Authors - Conghui Li
mouseWB

PLoS Biol

Loss of adenomatous polyposis coli function renders intestinal epithelial cells resistant to the cytokine IL-22.

Authors - Yu Chen
humanWB

Virulence

Inhibition of cell proliferation by Tas of foamy viruses through cell cycle arrest or apoptosis underlines the different mechanisms of virus-host interactions.

Authors - Wei Jie
humanWB

Biochem Pharmacol

PPARα regulates the expression of human arylacetamide deacetylase involved in drug hydrolysis and lipid metabolism.

Authors - Tomomi Morikawa
humanWB

Front Oncol

TFEB Promotes Prostate Cancer Progression via Regulating ABCA2-Dependent Lysosomal Biogenesis.

Authors - Xuejin Zhu
humanWB

Mol Cancer Res

Identification of Endogenous Adenomatous Polyposis Coli Interaction Partners and β-Catenin-Independent Targets by Proteomics.

Authors - Olesja Popow