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TBP Antibody 3 Publications

Mouse Monoclonal| Catalog number: 66166-1-Ig |CloneNo.: 2H3B2

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Con: 225 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

TBP Antibody FC HepG2 cells 66166-1-Ig

1X10^6 HepG2 cells were stained with 0.20ug TBP antibody (66166-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH

TBP Antibody WB HEK-293 cells 66166-1-Ig

Western blot analysis of TBP in various cell lines using Proteintech antibody 66166-1-Ig at a dilution of 1:8000 incubated at room temperature for 1.5 hours

TBP Antibody IP HEK-293 cells 66166-1-Ig

IP Result of anti-TBP (IP:66166-1-Ig, 3ug; Detection:66166-1-Ig 1:800) with HEK-293 cells lysate 1800ug.

TBP Antibody IHC human liver cancer tissue 66166-1-Ig

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 66166-1-Ig( TBP Antibody) at dilution of 1:650 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

TBP Antibody IF HeLa cells 66166-1-Ig

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 66166-1-Ig(TBP antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
human, mouse, rat, pig
Positive WB detected in:
HEK-293 cells, pig liver tissue
Positive IP detected in:
HEK-293 cells
Positive IHC detected in:
human liver cancer tissue, human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Positive FC detected in:
HepG2 cells
Recommended dilution:
WB : 1:2000-1:16000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:325-1:1300
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2a
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
TBP fusion protein Ag12383
Full name:
TATA box binding protein
Calculated molecular weight:
338aa,38 kDa
Observed molecular weight:
mouse/rat 33-36 kDa and human 37-43kDa
GenBank accession number:
BC110341
Gene ID (NCBI):
6908
Gene symbol
TBP
Synonyms
GTF2D, GTF2D1, SCA17, TATA binding factor, TATA box binding protein, TATA box factor, TATA sequence binding protein, TBP, TF2D, TFIID

Background

The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody detects human TBP (~40 kDa) and mouse/rat Tbp (~35 kDa).


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