TBP Monoclonal antibody

TBP Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2H3B2

Cat no : 66166-1-Ig

Synonyms

GTF2D, GTF2D1, SCA17, TATA binding factor, TATA box binding protein, TATA box factor, TATA sequence binding protein, TBP, TF2D, TFIID


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  • Western Blot (WB) analysis of various lysates using TBP Monoclonal antibody (66166-1-Ig)

    WB analysis using 66166-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66166-1-Ig (TBP antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of various lysates using TBP Monoclonal antibody (66166-1-Ig)

    WB analysis using 66166-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66166-1-Ig (TBP antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using TBP Monoclonal antibody (66166-1-Ig)

    WB analysis of HEK-293 using 66166-1-Ig

    Western blot analysis of TBP in various cell lines using Proteintech antibody 66166-1-Ig at a dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using TBP Monoclonal antibody (66166-1-Ig)

    WB analysis of pig liver using 66166-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 66166-1-Ig (TBP Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using TBP Monoclonal antibody (66166-1-Ig)

    IP experiment of HEK-293 using 66166-1-Ig

    IP Result of anti-TBP (IP:66166-1-Ig, 3ug; Detection:66166-1-Ig 1:800) with HEK-293 cells lysate 1800ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TBP Monoclonal antibody (66166-1-Ig)

    IHC staining of human liver cancer using 66166-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66166-1-Ig (TBP Antibody) at dilution of 1:650 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TBP Monoclonal antibody (66166-1-Ig)

    IHC staining of human breast cancer using 66166-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66166-1-Ig (TBP Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TBP Monoclonal antibody (66166-1-Ig)

    IHC staining of human breast cancer using 66166-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66166-1-Ig (TBP Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TBP Monoclonal antibody (66166-1-Ig)

    IHC staining of human breast cancer using 66166-1-Ig

    After antigen unmasking with Tris-EDTA buffer (pH 9.0) for 15 mins and 2M HCL treatment for 30mins, Immunohistochemistry of paraffin-embedded human breast cancer tissue slideusing 66166-1-Ig (TBP Antibody) at dilution of 1:400 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TBP Monoclonal antibody (66166-1-Ig)

    IHC staining of human breast cancer using 66166-1-Ig

    I After antigen unmasking with Tris-EDTA buffer (pH 9.0) for 15 mins and 2M HCL treatment for 30mins, Immunohistochemistry of paraffin-embedded human breast cancer tissue slideusing 66166-1-Ig (TBP Antibody) at dilution of 1:400 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TBP Monoclonal antibody (66166-1-Ig)

    IHC staining of human liver cancer using 66166-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66166-1-Ig (TBP Antibody) at dilution of 1:650 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using TBP Monoclonal antibody (66166-1-Ig)

    IF Staining of A431 using 66166-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TBP antibody (66166-1-Ig, Clone: 2H3B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TBP Monoclonal antibody (66166-1-Ig)

    IF Staining of HeLa using 66166-1-Ig

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66166-1-Ig(TBP antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using TBP Monoclonal antibody (66166-1-Ig)

    FC experiment of HepG2 using 66166-1-Ig

    1X10^6 HepG2 cells were stained with 0.20ug TBP antibody (66166-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

"TBP Antibodies" Comparison

View side-by-side comparison of TBP antibodies from other vendors to find the one that best suits your research needs.

Tested Applications

Positive WB detected inLNCaP cells, HSC-T6 cells, HEK-293 cells, pig liver tissue, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, Neuro-2a cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells, HeLa cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:325-1:1300
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66166-1-Ig targets TBP in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TBP fusion protein Ag12383
Full Name TATA box binding protein
Calculated molecular weight 338 aa, 38 kDa
Observed molecular weight mouse/rat 33-36 kDa and human 37-43kDa
GenBank accession numberBC110341
Gene symbol TBP
Gene ID (NCBI) 6908
RRIDAB_2881562
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody detects human TBP (~40 kDa) and mouse/rat Tbp (~35 kDa).

Protocols

Product Specific Protocols
WB protocol for TBP antibody 66166-1-IgDownload protocol
IHC protocol for TBP antibody 66166-1-IgDownload protocol
IF protocol for TBP antibody 66166-1-IgDownload protocol
IP protocol for TBP antibody 66166-1-IgDownload protocol
FC protocol for TBP antibody 66166-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Genome Res

Ligand-induced native G-quadruplex stabilization impairs transcription initiation.

Authors - Conghui Li
mouseWB

PLoS Biol

Loss of adenomatous polyposis coli function renders intestinal epithelial cells resistant to the cytokine IL-22.

Authors - Yu Chen
humanWB

Virulence

Inhibition of cell proliferation by Tas of foamy viruses through cell cycle arrest or apoptosis underlines the different mechanisms of virus-host interactions.

Authors - Wei Jie
humanWB

Biochem Pharmacol

PPARα regulates the expression of human arylacetamide deacetylase involved in drug hydrolysis and lipid metabolism.

Authors - Tomomi Morikawa
humanWB

Front Oncol

TFEB Promotes Prostate Cancer Progression via Regulating ABCA2-Dependent Lysosomal Biogenesis.

Authors - Xuejin Zhu
humanWB

Mol Cancer Res

Identification of Endogenous Adenomatous Polyposis Coli Interaction Partners and β-Catenin-Independent Targets by Proteomics.

Authors - Olesja Popow