CoraLite® Plus 488-conjugated TBP Monoclonal antibody

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  IF Staining of C2C12 using CL488-66166

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CL488-66166 (TBP antibody) at dilution of 1:50.

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  IF Staining of A431 using CL488-66166

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 TBP antibody (CL488-66166, Clone: 2H3B2 ) at dilution of 1:100.

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  IF Staining of C2C12 using CL488-66166

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CoraLite® Plus 488-conjugated TBP antibody (CL488-66166, Clone: 2H3B2 ) at dilution of 1:100.

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  FC experiment of HeLa using CL488-66166

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human TBP (CL488-66166, Clone:2H3B2) (red), or 0.2 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

TBP Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL488-66166

Clone No.2H3B2

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Synonyms

TATA sequence binding protein, TATA box factor, TATA box binding protein, TATA binding factor, GTF2D1

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Tested Applications

• Positive IF/ICC detected in: C2C12 cells, A431 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66166 targets TBP in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12383

  Product name: Recombinant human TBP protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-338 aa of BC110341

  Sequence: MDQNNSLPPYAQGLASPQGAMTPGIPIFSPMMPYGTGLTPQPIQNTNSLSILEEQQRQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQAVAAAAVQQSTSQQATQGTSGQAPQLFHSQTLTTAPLPGTTPLYPSPMTPMTPITPATPASESSGIVPQLQNIVSTVNLGCKLDLKTIALRARNAEYNPKRFAAVIMRIREPRTTALIFSSGKMVCTGAKSEEQSRLAARKYARVVQKLGFPAKFLDFKIQNMVGSCDVKFPIRLEGLVLTHQQFSSYEPELFPGLIYRMIKPRIVLLIFVSGKVVLTGAKVRAEIYEAFENIYPILKGFRKTT

• Full Name: TATA box binding protein
• Calculated Molecular Weight: 338 aa, 38 kDa
• GenBank Accession Number: BC110341
• Gene Symbol: TBP
• Gene ID (NCBI): 6908
• RRID: AB_2883275
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P20226
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody detects human TBP (~40 kDa) and mouse/rat Tbp (~35 kDa).