TBRG4 Polyclonal antibody

TBRG4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10177-2-AP

Synonyms

Protein TBRG4, KIAA0948, FASTKD4, FAST kinase domain-containing protein 4, CPR2

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Validation Data Gallery

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  WB analysis of A431 using 10177-2-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10177-2-AP (TBRG4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10177-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10177-2-AP (TBRG4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 10177-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10177-2-AP (TBRG4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10177-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10177-2-AP (TBRG4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10177-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10177-2-AP (TBRG4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10177-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TBRG4 antibody (10177-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: A431 cells, HepG2 cells
• Positive IHC detected in: human stomach cancer tissue, human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10177-2-AP targets TBRG4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TBRG4 fusion protein Ag0212
• Full Name: transforming growth factor beta regulator 4
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC002732
• Gene Symbol: TBRG4
• Gene ID (NCBI): 9238
• RRID: AB_2255699
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.