TCEB1 Monoclonal antibody, PBS Only

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• TCEB1 Monoclonal antibody, PBS Only (68164-1-PBS)

TCEB1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 68164-1-PBS Copied!

Clone No. 2D6D8

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Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

2D6D8, EloC, Elongin 15 kDa subunit, Elongin C, Elongin-C

• 

  WB analysis using 68164-1-Ig (same clone as 68164-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68164-1-Ig (TCEB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68164-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of T-47D using 68164-1-Ig (same clone as 68164-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed T-47D cells using TCEB1 antibody (68164-1-Ig, Clone: 2D6D8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68164-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

68164-1-PBS targets TCEB1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22601

  Product name: Recombinant human TCEB1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-112 aa of BC013809

  Sequence: MDGEEKTYGGCEGPDAMYVKLISSDGHEFIVKREHALTSGTIKAMLSGPGQFAENETNEVNFREIPSHVLSKVCMYFTYKVRYTNSSTEIPEFPIAPEIALELLMAANFLDC

• Full Name: transcription elongation factor B (SIII), polypeptide 1 (15kDa, elongin C)
• Calculated Molecular Weight: 112 aa, 12 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC013809
• Gene Symbol: TCEB1
• Gene ID (NCBI): 6921
• RRID: AB_2923683
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15369
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TCEB1, also named as RNA polymerase II transcription factor SIII subunit C, belongs to the SKP1 family. It mediates the ubiquitination of ECS-E3 ubiquitin-protein ligase complexes. TCEB1 is overexpressed in prostate cancer cell line PC-3 and breast cancer cell line SK-BR-3. TCEB1 also promotes invasion of prostate cancer cells (PMID:18844214).