TCP1 Polyclonal antibody

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  WB analysis using 10320-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10320-1-AP (TCP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10320-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10320-1-AP (TCP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10320-1-AP

  IP result of anti-TCP1 (IP:10320-1-AP, 4ug; Detection:10320-1-AP 1:300) with HeLa cells lysate 1600ug.

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  IHC staining of human colon cancer using 10320-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10320-1-AP (TCP1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 10320-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 10320-1-AP (TCP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 10320-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 10320-1-AP (TCP1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 10320-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10320-1-AP (TCP1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 10320-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10320-1-AP (TCP1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 10320-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10320-1-AP (TCP1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 10320-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10320-1-AP (TCP1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 10320-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TCP1 antibody (10320-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 10320-1-AP

  Immunofluorescent analysis of MCF-7 cells using 10320-1-AP (TCP1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 10320-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug TCP1 Polyclonal antibody (10320-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

TCP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10320-1-AP

Publications(11)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

CCT alpha, CCT1, CCTa, CCT-alpha, Chaperonin containing T-complex polypeptide 1 subunit 1

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Tested Applications

• Positive WB detected in: A549 cells, mouse heart tissue, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, NCI-H1299 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue, human colon tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, MCF-7 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:800-1:3200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Product Information

10320-1-AP targets TCP1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0389

  Product name: Recombinant human TCP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 276-553 aa of BC000665

  Sequence: QKILATGANVILTTGGIDDMCLKYFVEAGAMAVRRVLKRDLKRIAKASGATILSTLANLEGEETFEAAMLGQAEEVVQERICDDELILIKNTKARTSASIILRGANDFMCDEMERSLHDALCVVKRVLESKSVVPGGGAVEAALSIYLENYATSMGSREQLAIAEFARSLLVIPNTLAVNAAQDSTDLVAKLRAFHNEAQVNPERKNLKWIGLDLSNGKPRDNKQAGVFEPTIVKVKSLKFATEAAITILRIDDLIKLHPESKDDKHGSYEDAVHSGA

• Full Name: t-complex 1
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC000665
• Gene Symbol: TCP1
• Gene ID (NCBI): 6950
• RRID: AB_10694136
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P17987
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

0

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell; Mechanism of orphan subunit recognition during assembly quality control; Authors - Yuichi Yagita
human	WB	Sci Adv; EIF3D safeguards the homeostasis of key signaling pathways in human primed pluripotency; Authors - Chikako Okubo
human	WB	J Cell Biol; CEP55 promotes cilia disassembly through stabilizing Aurora A kinase.; Authors - Yu-Cheng Zhang
mouse,human	WB	Front Cell Dev Biol; TRiC/CCT chaperonin is required for the folding and inhibitory effect of WDTC1 on adipogenesis; Authors - Wen-Shuai Tang
mouse	WB	J Alzheimers Dis; Aging as a Precipitating Factor in Chronic Restraint Stress-Induced Tau Aggregation Pathology, and the Protective Effects of Rosmarinic Acid; Authors - Ye Shan
mouse	IP	Virology; Murine cytomegalovirus M72 promotes acute virus replication in vivo and is a substrate of the TRiC/CCT complex.; Authors - Sandhya Gopal