TEKT1 Recombinant monoclonal antibody

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  WB analysis using 87530-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87530-1-RR (TEKT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  Affinity of 87530-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87530-1-RR against Human TEKT1 were performed. The affinity constant is below 1 pM.

TEKT1 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87530-1-RR

Clone No.252780E12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Tektin-1

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87530-1-RR targets TEKT1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag5173

  Product name: Recombinant human TEKT1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 118-418 aa of BC014599

  Sequence: YREKRIGIDLVHDTVEHELIKEAEIIQGIMALLTRTLEEASEQIRMNRSAKYNLEKDLKDKFVALTIDDICFSLNNNSPNIRYSENAVRIEPNSVSLEDWLDFSSTNVEKADKQRNNSLMLKALVDRILSQTANDLRKQCDVVDTAFKNGLKDTKDARDKLADHLAKVMEEIASQEKNITALEKAILDQEGPAKVAHTRLETRTHRPNVELCRDVAQYRLMKEVQEITHNVARLKETLAQAQAELKGLHRRQLALQEEIQVKENTIYIDEVLCMQMRKSIPLRDGEDHGVWAGGLRPDAVC

• Full Name: tektin 1
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48-50 kDa
• GenBank Accession Number: BC014599
• Gene Symbol: TEKT1
• Gene ID (NCBI): 83659
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q969V4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TEKT1 belongs to the tektin family. Tektins comprise a family of filament-forming proteins that are coassembled with tubulins to form ciliary and flagellar microtubules. TEKT1 has 4 evolutionarily conserved cysteines and a tektin-specific peptide sequence. TEKT1 is predominantly expressed in the testis.