TEKT2 Recombinant monoclonal antibody

• 

  WB analysis using 87634-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87634-1-RR (TEKT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

TEKT2 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87634-1-RR

Clone No.252977C1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

H-tektin-T, TEKTB1, Tektin 2, Tektin B1, Tektin-2

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue, human testis tissue, mouse ovary tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87634-1-RR targets TEKT2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag4457

  Product name: Recombinant human TEKT2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-430 aa of BC035620

  Sequence: MATLSVKPSRRFQLPDWHTNSYLLSTNAQLQRDASHQIRQEARVLRNETNNQTIWDEHDNRTRLVERIDTVNRWKEMLDKCLTDLDAEIDALTQMKESAEQNLQAKNLPLDVAIECLTLRESRRDIDVVKDPVEDELHKEVEVIEATKKALQQKVSQAFEQLCLLQEVQQQLNSDHRGKMETLEIDRGCLSLNLRSPNISLKVDPTRVPDGSTTLQQWDDFSRFNKDRAEAEMKAATELREATALTIAETNNELEAQRVATEFAFRKRLREMEKVYSELKWQEKNTLEEIAELQEDIRHLEEDLRTKLLSLKLSHTRLEARTYRPNVELCRDQAQYGLTDEVHQLEATIAALKQKLAQAQDALDALCKHLARLQADIACKANSMLLDTKCMDTRRKLTVPAERFVPEVDTFTRTTNSTLSPLKSCQLELA

• Full Name: tektin 2 (testicular)
• Calculated Molecular Weight: 430 aa, 50 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC035620
• Gene Symbol: TEKT2
• Gene ID (NCBI): 27285
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UIF3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TEKT2 belongs to the tektin family. Tektins comprise a family of filament-forming proteins that are coassembled with tubulins to form ciliary and flagellar microtubules. TEKT2 and other members of the tektin protein family form filamentous polymers in the walls of ciliary and flagellar microtubules. Western blot analysis of testis showed a TEKT2 protein with an apparent molecular mass of 54 kDa (PMID: 12029069).