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TELO2 Polyclonal antibody

TELO2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15975-1-AP

Synonyms

CLK2, hCLK2, KIAA0683, Protein clk 2 homolog, TEL2, TELO2



Tested Applications

Positive WB detected inA431 cells, Y79 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15975-1-AP targets TELO2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TELO2 fusion protein Ag8763
Full Name TEL2, telomere maintenance 2, homolog (S. cerevisiae)
Calculated Molecular Weight 837 aa, 92 kDa
Observed Molecular Weight 92 kDa
GenBank Accession NumberBC017188
Gene Symbol TELO2
Gene ID (NCBI) 9894
RRIDAB_2203337
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TELO2 gene encodes a telomere length regulation protein TEL2 homolog. TELO2 may be involved in telomere length regulation and can form TTT complex with TTI1 and TTI2. TTT complex is required to stabilize protein levels of PIKK family proteins and is involved in the cellular resistance to DNA damage stresses, like ionizing radiation (IR), ultraviolet (UV) and MMC. The activity of mTORC1 and mTORC2 complexes, which regulate cell growth and survival in response to nutrient and hormonal signals, can be promoted, stabilized and maintained by TELO2.

Protocols

Product Specific Protocols
WB protocol for TELO2 antibody 15975-1-APDownload protocol
IHC protocol for TELO2 antibody 15975-1-APDownload protocol
IF protocol for TELO2 antibody 15975-1-APDownload protocol
IP protocol for TELO2 antibody 15975-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cell Biol

SCFFbxo9 and CK2 direct the cellular response to growth factor withdrawal via Tel2/Tti1 degradation and promote survival in multiple myeloma.

Authors - Fernández-Sáiz Vanesa V
humanWB

Mol Cell

The IMiD target CRBN determines HSP90 activity toward transmembrane proteins essential in multiple myeloma.

Authors - Michael Heider
mouseWB

Mol Cell

Metabolic stress controls mTORC1 lysosomal localization and dimerization by regulating the TTT-RUVBL1/2 complex.

Authors - Sang Gyun Kim
humanWB

Sci Adv

Synthetic lethality by targeting the RUVBL1/2-TTT complex in mTORC1-hyperactive cancer cells.

Authors - Seung Ho Shin
humanWB,IP

Genes Dev

A genetic screen identifies the Triple T complex required for DNA damage signaling and ATM and ATR stability.

Authors - Hurov Kristen E KE
humanWB

Am J Hum Genet

A Syndromic Intellectual Disability Disorder Caused by Variants in TELO2, a Gene Encoding a Component of the TTT Complex.

Authors - Jing You