Tested Applications
| Positive WB detected in | A431 cells, HL-60 cells, HEK-293 cells, HeLa cells, HepG2 cells, K-562 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21207-1-AP targets TET2 in WB, IHC, IF/ICC, FC (Intra), CoIP, ChIP, RIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, goat, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15583 Product name: Recombinant human TET2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC150180 Sequence: MEQDRTNHVEGNRLSPFLIPSPPICQTERLATKLQNGSPLPERAHPEVNGDTKWHSFKSYYGIPCMKGSQNSRVSPDFTQESRGYSKCLQNGGIKRTVSEPSLSGLLQIKKLKQDQKANGERRNFGVSQERNPGESSQPNVSDLSDKKESVSSVAQENAVKDFTSFSTHNCSGPENPELQILNEQEGKSANYHDKNIVLLKNKAVLMPNGATVSASSVEHTHGELLEKTLSQYYPDCVSIAVQKTTSHINAINSQATNELSCEITHPSHTSGQINSAQTSNSELPPKPAAVVSEACDADDADNASKLAAMLNTCSFQKPEQLQQQKSVFEICPSPAENNIQGTTKLASGE Predict reactive species |
| Full Name | tet oncogene family member 2 |
| Calculated Molecular Weight | 2002 aa, 224 kDa |
| Observed Molecular Weight | 130 kDa, 250 kDa |
| GenBank Accession Number | BC150180 |
| Gene Symbol | TET2 |
| Gene ID (NCBI) | 54790 |
| RRID | AB_10734584 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6N021 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TET2 is an enzyme which converts 5-methylcytosine (5-mC) to 5-hydroxymethylcytosine (5-hmC) in DNA and is important for normal myelopoiesis. TET2 mutations are frequently observed in myeloid malignancies, including myeloproliferative neoplasms. TET2 is broadly expressed in a variety of tissues. In healthy tissues, TET2 expression was shown to be elevated in hematopoietic cells with highest expression in granulocytes. Three isoforms of TET2 exists due to the alternative splicing. 220-250 kDa bands represent the full-length isoform; 130-133 kDa bands represent the nuclear isoforms of TET2. (PMID: 24615693) This antibody recognizes all three isoforms of TET2.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for TET2 antibody 21207-1-AP | Download protocol |
| IF protocol for TET2 antibody 21207-1-AP | Download protocol |
| IHC protocol for TET2 antibody 21207-1-AP | Download protocol |
| WB protocol for TET2 antibody 21207-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TET2 Polyclonal antibody (Cat# 21207-1-AP) has 99 citations. These appear in high-impact journals including Cancer Discovery, Nature Metabolism, Nature Communications, Science Advances, Cell Reports, Cell Death & Disease, JCI Insight, PNAS, and Journal of Biological Chemistry, among others. Research fields span epigenetics and DNA methylation/demethylation, cancer (leukemia, melanoma, colorectal, hepatocellular), metabolism and diabetes, neurology, immunology, and cardiovascular disease, with applications mainly in WB, IF, IHC, ChIP, and CoIP.
| Species | Application | Title |
|---|---|---|
Cancer Discov CRISPR Dependency Screens in Primary Hematopoietic Stem Cells Identify KDM3B as a Genotype Specific Vulnerability in IDH2- and TET2-Mutant Cells | ||
Nat Metab Maternal exercise prevents metabolic disorders in offspring mice through SERPINA3C | ||
Protein Cell Non-catalytic roles for TET1 protein negatively regulating neuronal differentiation through srGAP3 in neuroblastoma cells. | ||
Nat Commun Redirected nuclear glutamate dehydrogenase supplies Tet3 with α-ketoglutarate in neurons. | ||
Nat Commun Photoactivatable CRISPR/Cas13d via upconversion nanoparticles for deep tissue RNA engineering and orthopedic therapy. | ||
Nat Commun A negative feedback loop between TET2 and leptin in adipocyte regulates body weight |
Reviews
What customers say
Both reviews rated the product 5 stars. One user reported successful IHC on 5 µm skin sections at 1:400 dilution, observing positive staining in the epithelial layer consistent with expected expression, using citrate buffer pH 6 for antigen retrieval. The other user confirmed strong performance in Western Blot at 1:1000 dilution on K562 cell lysates. Overall, customers found the antibody reliable and specific across both applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Melanie (Verified Customer) (11-19-2019) | IHC of 5um thin skin sections was performed. A positive staining was observed in the skin epithelial layer as predicted by the human protein atlas. Citrate buffer pH6 was used for antigen retrieval
 |
Sid (Verified Customer) (04-12-2019) | Product performed well in western blot
|
