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  • KD/KO Validated

Transferrin Polyclonal antibody

Transferrin Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17435-1-AP

Synonyms

Beta 1 metal binding globulin, PRO1557, PRO2086, Serotransferrin, Siderophilin, TF, Transferrin



Tested Applications

Positive WB detected inhuman urine sample, mouse kidney tissue, mouse spleen tissue, rat kidney tissue
Positive IHC detected inhuman hepatocirrhosis tissue, human placenta tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17435-1-AP targets Transferrin in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Transferrin fusion protein Ag11504
Full Name transferrin
Calculated molecular weight 698 aa, 77 kDa
Observed molecular weight 77 kDa
GenBank accession numberBC059367
Gene symbol TF
Gene ID (NCBI) 7018
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

What is the tissue specificity of TF?

Transferrin is synthesized mainly in the liver.

What is the calculated molecular weight of TF?

77 kDa 

How many iron atoms can bind to TF?

Each of the two lobes of TF has one high-affinity Fe(III) binding site. Since binding of iron and bicarbonate 

releases free H+ ions, affinity decreases in acidic conditions, in which TF also remains bound to its receptor.

What is the primary role of TF?

TF serves as a serum metal-binding protein, playing a key role in iron metabolism and delivery for erythropoiesis.

What is the role of TF in human diseases?

TF deficiency results in an exhibition of congenital atransferrinemia (hypotransferrinemia) in which there is a 

decrease in hepcidin expression, causing increased iron levels. 

Is TF essential for iron delivery?

Congenital atransferrinemia results in an iron overload in some tissues, such as the liver, suggesting that while  TF is not essential for iron delivery to all organs, it is needed for iron level regulation.

In what circumstances do TF levels decrease?

Inflammation and iron overload both can cause a decline in TF levels.

How does TF affect hormonal balance?

TF positively regulates expression of hepcidin, a peptide hormone synthesized by the liver. 

How does TF deficiency cause increased iron levels? 

TF regulates hepcidin, which in turn is a negative regulator of intestinal iron absorption, resulting in TF 

deficiency causing both anemia, and, paradoxically, increased iron levels in some tissues. 

PMIDs: 23046645, 9635730, 22294463, 24589273

Protocols

Product Specific Protocols
WB protocol for Transferrin antibody 17435-1-APDownload protocol
IHC protocol for Transferrin antibody 17435-1-APDownload protocol
IF protocol for Transferrin antibody 17435-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Ecotoxicol Environ Saf

PMID: 30974244

Authors - Qi Wang
mouseWB

J Biol Inorg Chem

Investigating the role of transferrin in the distribution of iron, manganese, copper, and zinc.

Authors - Carolina Herrera
mouseIHC

Immunol Lett

Lipid A-activated inducible nitric oxide synthase expression via nuclear factor-κB in mouse choroid plexus cells.

Authors - Masaoki Takano
mouseWB

Int J Mol Sci

Bisphenol A Deranges the Endocannabinoid System of Primary Sertoli Cells with an Impact on Inhibin B Production.

Authors - Gianna Rossi
ratWB

Exp Lung Res

NF-κB p65 Knock-down inhibits TF, PAI-1 and promotes activated protein C production in lipopolysaccharide-stimulated alveolar epithelial cells type II.

Authors - Bo Liu
mouseWB

Mol Med Rep

Increased expression levels of inflammatory cytokines and adhesion molecules in lipopolysaccharide‑induced acute inflammatory apoM‑/‑ mice.

Authors - Yuanping Shi