Transferrin Monoclonal antibody

Transferrin Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

2B4C6

Cat no : 66171-1-Ig

Synonyms

Beta 1 metal binding globulin, PRO1557, PRO2086, Serotransferrin, Siderophilin, TF, Transferrin

---

Validation Data Gallery

• 

  WB analysis of human plasma using 66171-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 66171-1-Ig (Transferrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 66171-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66171-1-Ig (Transferrin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66171-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66171-1-Ig (Transferrin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66171-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66171-1-Ig (Transferrin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66171-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66171-1-Ig (Transferrin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 66171-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66171-1-Ig (Transferrin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 66171-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66171-1-Ig (Transferrin antibody at dilution of 1:500 (under 10x lens).

• 

  IHC staining of human liver using 66171-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66171-1-Ig (Transferrin antibody at dilution of 1:500 (under 40x lens).

• 

  IF Staining of human liver using 66171-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Transferrin antibody (66171-1-Ig, Clone: 2B4C6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human liver using 66171-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Transferrin antibody (66171-1-Ig, Clone: 2B4C6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Tested Applications

• Positive WB detected in: human plasma, fetal human brain tissue
• Positive IHC detected in: human liver cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

66171-1-Ig targets Transferrin in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Transferrin fusion protein Ag11668
• Full Name: transferrin
• Calculated Molecular Weight: 698 aa, 77 kDa
• Observed Molecular Weight: 77 kDa
• GenBank Accession Number: BC059367
• Gene Symbol: TF
• Gene ID (NCBI): 7018
• RRID: AB_2881566
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

What is the tissue specificity of TF?

Transferrin is synthesized mainly in the liver.

What is the calculated molecular weight of TF?

77 kDa 

How many iron atoms can bind to TF?

Each of the two lobes of TF has one high-affinity Fe(III) binding site. Since binding of iron and bicarbonate 

releases free H+ ions, affinity decreases in acidic conditions, in which TF also remains bound to its receptor.

What is the primary role of TF?

TF serves as a serum metal-binding protein, playing a key role in iron metabolism and delivery for erythropoiesis.

What is the role of TF in human diseases?

TF deficiency results in an exhibition of congenital atransferrinemia (hypotransferrinemia) in which there is a 

decrease in hepcidin expression, causing increased iron levels. 

Is TF essential for iron delivery?

Congenital atransferrinemia results in an iron overload in some tissues, such as the liver, suggesting that while  TF is not essential for iron delivery to all organs, it is needed for iron level regulation.

In what circumstances do TF levels decrease?

Inflammation and iron overload both can cause a decline in TF levels.

How does TF affect hormonal balance?

TF positively regulates expression of hepcidin, a peptide hormone synthesized by the liver. 

How does TF deficiency cause increased iron levels? 

TF regulates hepcidin, which in turn is a negative regulator of intestinal iron absorption, resulting in TF 

deficiency causing both anemia, and, paradoxically, increased iron levels in some tissues. 

PMIDs: 23046645, 9635730, 22294463, 24589273

Publications

Species	Application	Title
human	WB	Cell Death Dis; Super-enhancer-driven MLX mediates redox balance maintenance via SLC7A11 in osteosarcoma; Authors - Weitang Guo
human	WB	Eur Rev Med Pharmacol Sci; Decreased serum SIRT6 as a novel predictor of coronary artery disease; Authors - Z. YAN
	WB	bioRxiv; Progranulin inhibits phospholipase sPLA2-IIA to control neuroinflammation; Authors - Huan Du
	WB	Neurochem Int; Quantitative iTRAQ-based proteomic analysis of piperine protected cerebral ischemia/reperfusion injury in rat brain.; Authors - Yichun Zou