CoraLite®594-conjugated Transferrin Monoclonal antibody

Transferrin Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2B4C6

Cat no : CL594-66171

Synonyms

Beta 1 metal binding globulin, DKFZp781D0156, PRO1557, PRO2086, Serotransferrin, Siderophilin, TF, transferrin

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Validation Data Gallery

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  IF Staining of human liver cancer using CL594-66171

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 Transferrin antibody (CL594-66171, Clone: 2B4C6 ) at dilution of 1:100.

• 

  IF Staining of human liver cancer using CL594-66171

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 Transferrin antibody (CL594-66171, Clone: 2B4C6 ) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: human liver cancer tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66171 targets Transferrin in IF applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Transferrin fusion protein Ag11668
• Full Name: transferrin
• Calculated Molecular Weight: 698 aa, 77 kDa
• Observed Molecular Weight: 77 kDa
• GenBank Accession Number: BC059367
• Gene Symbol: TF
• Gene ID (NCBI): 7018
• RRID: AB_2919957
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

What is the tissue specificity of TF?

Transferrin is synthesized mainly in the liver.

What is the calculated molecular weight of TF?

77 kDa 

How many iron atoms can bind to TF?

Each of the two lobes of TF has one high-affinity Fe(III) binding site. Since binding of iron and bicarbonate 

releases free H+ ions, affinity decreases in acidic conditions, in which TF also remains bound to its receptor.

What is the primary role of TF?

TF serves as a serum metal-binding protein, playing a key role in iron metabolism and delivery for erythropoiesis.

What is the role of TF in human diseases?

TF deficiency results in an exhibition of congenital atransferrinemia (hypotransferrinemia) in which there is a 

decrease in hepcidin expression, causing increased iron levels. 

Is TF essential for iron delivery?

Congenital atransferrinemia results in an iron overload in some tissues, such as the liver, suggesting that while  TF is not essential for iron delivery to all organs, it is needed for iron level regulation.

In what circumstances do TF levels decrease?

Inflammation and iron overload both can cause a decline in TF levels.

How does TF affect hormonal balance?

TF positively regulates expression of hepcidin, a peptide hormone synthesized by the liver. 

How does TF deficiency cause increased iron levels? 

TF regulates hepcidin, which in turn is a negative regulator of intestinal iron absorption, resulting in TF 

deficiency causing both anemia, and, paradoxically, increased iron levels in some tissues. 

PMIDs: 23046645, 9635730, 22294463, 24589273