Transferrin Monoclonal antibody, PBS Only

Transferrin Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B4C6

Cat no : 66171-1-PBS

Synonyms

Beta 1 metal binding globulin, PRO1557, PRO2086, Serotransferrin, Siderophilin, TF, Transferrin

---

Validation Data Gallery

• 

  WB analysis of human plasma using 66171-1-Ig (same clone as 66171-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 66171-1-Ig (Transferrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 66171-1-Ig (same clone as 66171-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66171-1-Ig (Transferrin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66171-1-Ig (same clone as 66171-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66171-1-Ig (Transferrin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66171-1-Ig (same clone as 66171-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66171-1-Ig (Transferrin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66171-1-Ig (same clone as 66171-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66171-1-Ig (Transferrin antibody at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66171-1-Ig (same clone as 66171-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66171-1-Ig (Transferrin antibody at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66171-1-Ig (same clone as 66171-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66171-1-Ig (Transferrin antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66171-1-Ig (same clone as 66171-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66171-1-Ig (Transferrin antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver using 66171-1-Ig (same clone as 66171-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Transferrin antibody (66171-1-Ig, Clone: 2B4C6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver using 66171-1-Ig (same clone as 66171-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Transferrin antibody (66171-1-Ig, Clone: 2B4C6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66171-1-PBS targets Transferrin in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Transferrin fusion protein Ag11668
• Full Name: transferrin
• Calculated Molecular Weight: 698 aa, 77 kDa
• Observed Molecular Weight: 77 kDa
• GenBank Accession Number: BC059367
• Gene Symbol: TF
• Gene ID (NCBI): 7018
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Transferrin(TF) is also named as Beta-1 metal-binding globulin, Siderophilin and belongs to the transferrin family. It is a bilobal iron-binding protein that plays the crucial role of binding ferric iron and keeping it in solution, thereby controlling the levels of this important metal. Serum transferrin may have a further role in stimulating cell proliferation.