NeutraKine® TGF beta 1 Monoclonal antibody

NeutraKine® TGF beta 1 Monoclonal Antibody for FC, IHC, Neutralization, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

Neutralization, IHC, FC, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

1E3E9

Cat no : 69012-1-Ig

Synonyms

CED, DPD1, LAP, NeutraKine® TGF beta 1, TGF ?1, TGF beta 1, TGFB, TGFB1, TGFbeta, TGF-beta1

---

Validation Data Gallery

• 

  Neutralization experiment of NeutraKine® TGF beta 1 using 69012-1-Ig

  Recombinant human TGF beta 1 (Cat.NO. HZ-1011) inhibits IL-4 induced proliferation of mouse HT-2 cells in a dose dependent nammer (blue curve, refer to bottom X-left Y). The activity of human TGF beta 1 (0.5 ng/mL HZ-1011) is neutralized by mouse anti-human TGF beta 1 antibody 69012-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 1-5 μg/mL.The NeutraControl mouse anti-human TGF beta 1 monoclonal antibody 69512-1-Ig could recognize human TGF beta 1, but could not neutralize human TGF beta 1 in the range of 0.0061-100 μg/mL(green curve, refer to top X-right Y).

• 

  IHC staining of human prostate cancer using 69012-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 69012-1-Ig (NeutraKine® TGF beta 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 69012-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 69012-1-Ig (NeutraKine® TGF beta 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HEK-293 using 69012-1-Ig

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human NeutraKine® TGF beta 1 (69012-1-Ig, Clone:1E3E9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  ELISA experiment of Recombinant protein using 69012-1-Ig

  Indirect ELISA was carried out by coating recombinant Human TGF beta 1 (Cat.NO. HZ-1011) at 70 ng/well followed by blocking and adding serial diluted TGF beta 1 antibody 69012-1-Ig and 69512-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

---

Neutralization

Product Information

69012-1-Ig targets NeutraKine® TGF beta 1 in Neutralization, IHC, FC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: human Humankine TGF beta 1 protein HZ-1011
• Full Name: transforming growth factor, beta 1
• Gene Symbol: TGFB1
• Gene ID (NCBI): 7040
• RRID: AB_2882930
• Conjugate: Unconjugated
• Form: Lyophilized Powder
• Purification Method: Protein A purification
• Storage Buffer: Sterile PBS.
• Endotoxin: <0.1 EU/μg
• Reconstitution: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

TGFB, also named as LAP and TGFB1, is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for it. TGFB positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. It is highly expressed in bone. Mutation of TGFB are the cause of Camurati-Engelmann disease (CED) which known as progressive diaphyseal dysplasia 1 (DPD1).

This antibody can be used to neutralize the bioactivity of TGF beta 1.

Publications

Species	Application	Title
human	Neutralization	Bioengineered; Emodin suppresses hepatocellular carcinoma growth by regulating macrophage polarization via microRNA-26a/transforming growth factor beta 1/protein kinase B.; Authors - Jiao Yin
human	Neutralization	Cell Death Discov; A positive feedback loop: RAD18-YAP-TGF-β between triple-negative breast cancer and macrophages regulates cancer stemness and progression.; Authors - Xueqi Yan
		Nature; Co-transplantation of autologous Treg cells in a cell therapy for Parkinson's disease; Authors - Tae-Yoon Park
		Stem Cell Res Ther; Blockade of TGF-β signalling alleviates human adipose stem cell senescence induced by native ECM in obesity visceral white adipose tissue; Authors - Xueya Han