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TGM2 Polyclonal antibody
TGM2 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IHC, IF, ELISA
Cat no : 15100-1-AP
Validation Data Gallery
|Positive WB detected in||C2C12 cells, HepG2 cells, HUVEC cells, HeLa cells, SGC-7901 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 3 publications below|
|WB||See 14 publications below|
|IHC||See 3 publications below|
|IF||See 2 publications below|
15100-1-AP targets TGM2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TGM2 fusion protein Ag7439|
|Full Name||transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)|
|Calculated molecular weight||77 kDa|
|Observed molecular weight||77-80 kDa|
|GenBank accession number||BC003551|
|Gene ID (NCBI)||7052|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
|Product Specific Protocols|
|WB protocol for TGM2 antibody 15100-1-AP||Download protocol|
|IHC protocol for TGM2 antibody 15100-1-AP||Download protocol|
|IP protocol for TGM2 antibody 15100-1-AP||Download protocol|
|Click here to view our Standard Protocols|
SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes
Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.
Front Endocrinol (Lausanne)
A long non-coding RNA that harbors a SNP associated with type 2 diabetes regulates the expression of TGM2 gene in pancreatic beta cells
AFF1 inhibits adipogenic differentiation via targeting TGM2 transcription.
Cancer Cell Int
Immune subtyping for pancreatic cancer with implication in clinical outcomes and improving immunotherapy.
Biochim Biophys Acta Mol Cell Res
TGM2 positively regulates myoblast differentiation via enhancing the mTOR signaling.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Jon (Verified Customer) (02-15-2022)
DILuted as suggested in %5 milk TBST blocking buffer. We have had problems with other TGM2 antibodies from other companies, but this one seemed no work better and yield a better signal. However, the apparent molecular weight of the TGM2 band appeared to be at a much lower MW than it is supposed to.