TGM2 Polyclonal antibody

TGM2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 15100-1-AP

Synonyms

G ALPHA h, GNAH, TG(C), TG2, TGase 2, TGase C, TGase H, TGC, TGM2, Tissue transglutaminase, Transglutaminase 2, Transglutaminase C, Transglutaminase H



Tested Applications

Positive WB detected inC2C12 cells, SGC-7901 cells, HUVEC cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15100-1-AP targets TGM2 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TGM2 fusion protein Ag7439
Full Name transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)
Calculated molecular weight 77 kDa
Observed molecular weight 77 kDa, 82 kDa
GenBank accession numberBC003551
Gene symbol TGM2
Gene ID (NCBI) 7052
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TGM2 antibody 15100-1-APDownload protocol
IHC protocol for TGM2 antibody 15100-1-APDownload protocol
IP protocol for TGM2 antibody 15100-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Sci

TGFβ-1 Induced Cross-Linking of the Extracellular Matrix of Primary Human Dermal Fibroblasts.

Authors - Mariya E Semkova
humanWB

J Exp Clin Cancer Res

Targeting SERT promotes tryptophan metabolism: mechanisms and implications in colon cancer treatment.

Authors - Di Ye
ratWB

Food Funct

Calcium relieves fluoride-induced bone damage through the PI3K/AKT pathway.

Authors - Jinming Wang
humanIHC

Sci Rep

Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer.

Authors - Katsutaka Mitachi
humanIHC,WB

Cancer Cell Int

Immune subtyping for pancreatic cancer with implication in clinical outcomes and improving immunotherapy.

Authors - Jingkai Liu
mouseWB

Cell Prolif

AFF1 inhibits adipogenic differentiation via targeting TGM2 transcription.

Authors - Yaqian Chen