Validation Data Gallery
|Positive WB detected in||C2C12 cells, HUVEC cells, HepG2 cells, BxPC-3 cells, HeLa cells, A549 cells, K-562 cells, SGC-7901 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15100-1-AP targets TGM2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TGM2 fusion protein Ag7439|
|Full Name||transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)|
|Calculated molecular weight||77 kDa|
|Observed molecular weight||77-80 kDa|
|GenBank accession number||BC003551|
|Gene ID (NCBI)||7052|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Cancer Commun (Lond)
Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer
SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes
Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.
Cell Oncol (Dordr)
Transglutaminases are oncogenic biomarkers in human cancers and therapeutic targeting of TGM2 blocks chemoresistance and macrophage infiltration in pancreatic cancer
Int J Mol Sci
TGFβ-1 Induced Cross-Linking of the Extracellular Matrix of Primary Human Dermal Fibroblasts.
Front Endocrinol (Lausanne)
A long non-coding RNA that harbors a SNP associated with type 2 diabetes regulates the expression of TGM2 gene in pancreatic beta cells
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Jon (Verified Customer) (02-15-2022)
DILuted as suggested in %5 milk TBST blocking buffer. We have had problems with other TGM2 antibodies from other companies, but this one seemed no work better and yield a better signal. However, the apparent molecular weight of the TGM2 band appeared to be at a much lower MW than it is supposed to.