• Featured Product
  • KD/KO Validated

TGM2 Polyclonal antibody

TGM2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15100-1-AP

Synonyms

G ALPHA h, GNAH, TG(C), TG2, TGase 2, TGase C, TGase H, TGC, TGM2, Tissue transglutaminase, Transglutaminase 2, Transglutaminase C, Transglutaminase H



Tested Applications

Positive WB detected inC2C12 cells, HepG2 cells, HUVEC cells, HeLa cells, SGC-7901 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15100-1-AP targets TGM2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TGM2 fusion protein Ag7439
Full Name transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)
Calculated molecular weight 77 kDa
Observed molecular weight 77-80 kDa
GenBank accession numberBC003551
Gene symbol TGM2
Gene ID (NCBI) 7052
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TGM2 antibody 15100-1-APDownload protocol
IHC protocol for TGM2 antibody 15100-1-APDownload protocol
IP protocol for TGM2 antibody 15100-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Sci

TGFβ-1 Induced Cross-Linking of the Extracellular Matrix of Primary Human Dermal Fibroblasts.

Authors - Mariya E Semkova
humanWB

J Exp Clin Cancer Res

Targeting SERT promotes tryptophan metabolism: mechanisms and implications in colon cancer treatment.

Authors - Di Ye
mouseWB

J Immunol Res

Propofol Suppresses Microglia Inflammation by Targeting TGM2/NF-κB Signaling.

Authors - Yuanyuan Hou
  • KD Validated
ratWB

Food Funct

Calcium relieves fluoride-induced bone damage through the PI3K/AKT pathway.

Authors - Jinming Wang
humanIHC

Sci Rep

Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer.

Authors - Katsutaka Mitachi
mouseWB,IF

Biochim Biophys Acta Mol Cell Res

TGM2 positively regulates myoblast differentiation via enhancing the mTOR signaling.

Authors - Dongdong Wang
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jon (Verified Customer) (02-15-2022)

DILuted as suggested in %5 milk TBST blocking buffer. We have had problems with other TGM2 antibodies from other companies, but this one seemed no work better and yield a better signal. However, the apparent molecular weight of the TGM2 band appeared to be at a much lower MW than it is supposed to.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: EndoCßH1 human pancreatic beta cell line
TGM2 Antibody Western Blot validation (1:1000 dilution) in EndoCßH1 human pancreatic beta cell line (Cat no:15100-1-AP)