TH Polyclonal antibody

TH Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25859-1-AP

Synonyms

TH, TYH, Tyrosine 3 hydroxylase, Tyrosine 3 monooxygenase, tyrosine hydroxylase



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, mouse heart tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue, SH-SY5Y cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25859-1-AP is TH Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, canine, Gerbil, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TH fusion protein Ag23075
Full Name tyrosine hydroxylase
Calculated molecular weight 528 aa, 59 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC104967
Gene symbol TH
Gene ID (NCBI) 7054
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of dopamine and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for dopaminergic and noradrenergic neurons. This protein has 6 isoforms produced by alternative splicing with the MW from 44 kDa to 58 kDa.

Protocols

Product Specific Protocols
WB protocol for TH antibody 25859-1-APDownload protocol
IHC protocol for TH antibody 25859-1-APDownload protocol
IF protocol for TH antibody 25859-1-APDownload protocol
IP protocol for TH antibody 25859-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IHC,IF

Front Cell Neurosci

PMID: 30555300

Authors - Cong Zheng
canineIF

Int J Cardiol

The effects of interleukin 17A on left stellate ganglion remodeling are mediated by neuroimmune communication in normal structural hearts.

Authors - Jielin Deng
mouseWB

Cell Death Dis

Microglia as modulators of exosomal alpha-synuclein transmission.

Authors - Yun Xia
mouseWB

Front Neurol

PMID: 30949126

Authors - Xingfang Guo
mouseWB,IHC

Front Cell Neurosci

TRH Analog, Taltirelin Protects Dopaminergic Neurons From Neurotoxicity of MPTP and Rotenone.

Authors - Cong Zheng
mouseWB

Mol Cell Biochem

Repetitive transcranial magnetic stimulation protects mice against 6-OHDA-induced Parkinson's disease symptoms by regulating brain amyloid β1-42 level.

Authors - Fang Ba

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Raphael (Verified Customer) (08-02-2021)

This antibody is good for western blot. The dilution at 1/1000 was too strong but at 1/2000 it works perfectly.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/2000
  • Cell Tissue Type: Rat brain
TH Antibody Western Blot validation (1/2000 dilution) in Rat brain (Cat no:25859-1-AP)
FH

Teresa (Verified Customer) (11-03-2017)

Standard protocol, blocked with 1% goat serum in TBS, heat-mediated antigen retrieval

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: wildtype mouse brain
TH Antibody Immunofluorescence validation (1:200 dilution) in wildtype mouse brain (Cat no:25859-1-AP)
FH

Teresa (Verified Customer) (10-16-2017)

Signal was strong and specific. Could probably dilute further.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: mouse brain
TH Antibody Immunohistochemistry validation (1:200 dilution) in mouse brain (Cat no:25859-1-AP)