THOC1 Polyclonal antibody

THOC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 10920-1-AP

Synonyms

Nuclear matrix protein p84, HPR1, Tho1, THO complex 1, Tho 1

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Validation Data Gallery

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  WB analysis of HEK-293 using 10920-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10920-1-AP (THOC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10920-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10920-1-AP (THOC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10920-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10920-1-AP (THOC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10920-1-AP

  IP result of anti-THOC1 (IP:10920-1-AP, 4ug; Detection:10920-1-AP 1:500) with HEK-293 cells lysate 1600 ug.

• 

  IHC staining of human colon cancer using 10920-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10920-1-AP (THOC1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HeLa using 10920-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using THOC1 antibody (10920-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 16 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

10920-1-AP targets THOC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: THOC1 fusion protein Ag1352
• Full Name: THO complex 1
• Calculated Molecular Weight: 84 kDa
• Observed Molecular Weight: 84 kDa
• GenBank Accession Number: BC010381
• Gene Symbol: THOC1
• Gene ID (NCBI): 9984
• RRID: AB_2202239
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

THOC1 (THO complex subunit 1), also known as Tho1, P84, HPR1 or P84N5, is a 657 amino acid nuclear matrix protein and is evolutionarily conserved from yeast to humans. THOC1 contains one death domain and is a component of the heteromultimeric THO/TREX (transcription/export) complex along with THOC2, THOC3, BAT1 and ALY. The THO/TREX complex is recruited to transcribed genes and travels along with RNA polymerase II (Pol II) during elongation, coupling elongating Pol II with RNA splicing and export factors. THOC1 is expressed at high levels in breast cancer cells and at relatively low levels in normal epithelia. A reduction of THOC1 in cancer cell lines results in promoted cancer cell apoptosis and reduced cell proliferation. This suggests that cancer cells are dependent on the high levels of THOC1 expression and therefore THOC1 may be a good target for cancer therapy. This antibody recognizes the 84kd THOC1 protein.

Publications

Species	Application	Title
human	WB	J Med Virol; SHFL inhibits enterovirus A71 infection by triggering degradation of viral 3Dpol protein via the ubiquitin-proteasome pathway; Authors - Chahui Tan
human	WB	Cancer Lett; TIMELESS confers cisplatin resistance in nasopharyngeal carcinoma by activating the Wnt/β-catenin signaling pathway and promoting the epithelial mesenchymal transition.; Authors - Sai-Lan Liu
mouse	WB	Elife; Post-transcriptional repression of circadian component CLOCK regulates cancer-stemness in murine breast cancer cells.; Authors - Takashi Ogino
human	WB,IF	Cancers (Basel); Cytoplasmic Clusterin Suppresses Lung Cancer Metastasis by Inhibiting the ROCK1-ERK Axis.; Authors - Shaobo Huang
	WB	Biochem Pharmacol; Modulation of cell physiology by bispecific nanobodies enabling changes in the intracellular localization of organelle proteins; Authors - Akito Tsuruta
human	WB	Cell Oncol (Dordr); Bioactive peptide inhibits acute myeloid leukemia cell proliferation by downregulating ALKBH5-mediated m6A demethylation of EIF4EBP1 and MLST8 mRNA.; Authors - Le Zhang