TICAM2 Recombinant monoclonal antibody

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  IHC staining of mouse testis using 84064-6-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84064-6-RR (TICAM2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 84064-6-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using TICAM2 antibody (84064-6-RR, Clone: 241186C12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

TICAM2 Uni-rAb^® Recombinant Antibody for IHC, IF/ICC, ELISA

Cat No. 84064-6-RR

Clone No.241186C12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

241186C12, MyD88-4, Putative NF-kappa-B-activating protein 502, TICAM 2, TICAM-2

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Tested Applications

• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84064-6-RR targets TICAM2 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: toll-like receptor adaptor molecule 2
• Calculated Molecular Weight: 27 kDa
• GenBank Accession Number: NM_021649
• Gene Symbol: TICAM2
• Gene ID (NCBI): 353376
• RRID: AB_3671634
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q86XR7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TICAM2, also named TIRAP3, TIRP, and TRAM, is involved in IL1-triggered NF-kappa-B activation, functioning upstream of IRAK1, IRAK2, TRAF6, and IKBKB. Physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1. TICAM2 is involved in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS.