TK1 Polyclonal antibody

• Featured Product
• KD/KO Validated

TK1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 15691-1-AP

Synonyms

TK2, Tk1b, Tk1a, TK 2, TK 1

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Validation Data Gallery

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  WB analysis using 15691-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15691-1-AP (TK1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15691-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15691-1-AP (TK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 15691-1-AP

  IP result of anti-TK1 (IP:15691-1-AP, 4ug; Detection:15691-1-AP 1:800) with HEK-293 cells lysate 1400 ug.

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  IHC staining of human breast cancer using 15691-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15691-1-AP (TK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15691-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15691-1-AP (TK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15691-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15691-1-AP (TK1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 15691-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15691-1-AP (TK1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of U2OS using 15691-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using TK1 antibody (15691-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, MCF-7 cells, SW 1990 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

15691-1-AP targets TK1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TK1 fusion protein Ag8249
• Full Name: thymidine kinase 1, soluble
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC007986
• Gene Symbol: TK1
• Gene ID (NCBI): 7083
• RRID: AB_2203613
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TK1(Thymidine kinase, cytosolic) belongs to the thymidine kinase family and is a regulatory enzyme that can interchange between its dimeric and tetrameric forms, which have low and high catalytic efficiencies, respectively. TK1 is involved in DNA synthesis and an important proliferation marker(PMID:21178264).

Publications

Species	Application	Title
human,mouse	WB,IHC	Cell Metab; Thymidine kinase 1 drives hepatocellular carcinoma in enzyme-dependent and -independent manners; Authors - Qing Li ; KD Validated
human	WB	Clin Transl Med; Parkin regulates IGF2BP3 through ubiquitination in the tumourigenesis of cervical cancer; Authors - Xin Sun
rat	IF	Theranostics; Intravenous transplantation of olfactory ensheathing cells reduces neuroinflammation after spinal cord injury via interleukin-1 receptor antagonist.; Authors - Lijian Zhang
human	WB	Oxid Med Cell Longev; lncRNA IGF2-AS Regulates Nucleotide Metabolism by Mediating HMGA1 to Promote Pyroptosis of Endothelial Progenitor Cells in Sepsis Patients.; Authors - Guibin Liang
mouse,human	WB	Cancer Biol Ther; rAAV9-UPII-TK-EGFP can precisely transduce a suicide gene and inhibit the growth of bladder tumors.; Authors - Foyan Lian
mouse	WB	Adv Biol Regul; Fhit and Wwox loss-associated genome instability: A genome caretaker one-two punch.; Authors - Morgan S Schrock