TK1 Polyclonal antibody

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• KD/KO Validated

TK1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15691-1-AP Copied!

Publications (19)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

EC:2.7.1.21, TK 1, TK 2, Tk1a, Tk1b

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  WB analysis using 15691-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15691-1-AP (TK1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 15691-1-AP

  WB result of TK1 antibody (15691-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TK1 transfected HEK-293 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HEK-293 using 15691-1-AP

  IP result of anti-TK1 (IP:15691-1-AP, 4ug; Detection:15691-1-AP 1:800) with HEK-293 cells lysate 1400 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 15691-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15691-1-AP (TK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 15691-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15691-1-AP (TK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 15691-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15691-1-AP (TK1 Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 15691-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15691-1-AP (TK1 Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of U2OS using 15691-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using TK1 antibody (15691-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, MCF-7 cells, SW 1990 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15691-1-AP targets TK1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8249

  Product name: Recombinant human TK1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-234 aa of BC007986

  Sequence: MSCINLPTVLPGSPSKTRGQIQVILGPMFSGKSTELMRRVRRFQIAQYKCLVIKYAKDTRYSSSFCTHDRNTMEALPACLLRDVAQEALGVAVIGIDEGQFFPDIVEFCEAMANAGKTVIVAALDGTFQRKPFGAILNLVPLAESVVKLTAVCMECFREAAYTKRLGTEKEVEVIGGADKYHSVCRLCYFKKASGQPAGPDNKENCPVPGKPGEAVAARKLFAPQQILQCSPAN

• Full Name: thymidine kinase 1, soluble
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC007986
• Gene Symbol: TK1
• Gene ID (NCBI): 7083
• RRID: AB_2203613
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P04183
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TK1(Thymidine kinase, cytosolic) belongs to the thymidine kinase family and is a regulatory enzyme that can interchange between its dimeric and tetrameric forms, which have low and high catalytic efficiencies, respectively. TK1 is involved in DNA synthesis and an important proliferation marker(PMID:21178264).

Publications

What published studies show

Anti-TK1 (Thymidine Kinase 1) antibody (Cat# 15691-1-AP) has 19 citations across journals including Cell Metabolism, Nature Structural & Molecular Biology, PLoS Biology, Theranostics, British Journal of Cancer, Journal of Medicinal Chemistry, Clinical & Translational Medicine, and BMC Cancer. Research fields span hepatocellular carcinoma, glioblastoma, cervical and colorectal cancer, nucleotide metabolism, neuroinflammation, hypertension, radiation therapy, blood–brain barrier pharmacology, and genome instability.

Automatically generated from the 19 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB,IHC	Cell Metab; Thymidine kinase 1 drives hepatocellular carcinoma in enzyme-dependent and -independent manners; Authors - Qing Li ; KD Validated
rat	IF	Theranostics; Intravenous transplantation of olfactory ensheathing cells reduces neuroinflammation after spinal cord injury via interleukin-1 receptor antagonist.; Authors - Lijian Zhang
human	IHC	Nat Struct Mol Biol; ERK-USP9X-coupled regulation of thymidine kinase 1 promotes both its enzyme activity-dependent and its enzyme activity-independent functions for tumor growth; Authors - Jingjing Tao
human	WB	Clin Transl Med; Parkin regulates IGF2BP3 through ubiquitination in the tumourigenesis of cervical cancer; Authors - Xin Sun
human	WB	Br J Cancer; Proteomics analysis and immune profiling reveal regulators of PD-L1 in oesophageal squamous cell carcinoma; Authors - Yuying Li
human	WB	Oxid Med Cell Longev; lncRNA IGF2-AS Regulates Nucleotide Metabolism by Mediating HMGA1 to Promote Pyroptosis of Endothelial Progenitor Cells in Sepsis Patients.; Authors - Guibin Liang