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  • KD/KO Validated

TLE3 Polyclonal antibody

TLE3 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 11372-1-AP


ESG, ESG3, GRG3, HsT18976, KIAA1547, TLE3

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11372-1-AP targets TLE3 in WB, IP, IF, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TLE3 fusion protein Ag1930
Full Name transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
Calculated Molecular Weight 772 aa, 83 kDa
Observed Molecular Weight 83 kDa
GenBank Accession NumberBC015729
Gene Symbol TLE3
Gene ID (NCBI) 7090
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TLE3, also named as KIAA1547 and ESG3, belongs to the WD repeat Groucho/TLE family. It is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. TLE3 is a member of the transducin-like enhancer of split (TLE) family of proteins that have been implicated in the tumorgenesis and classification of sarcomas. It is a candidate biomarker of response to taxane


Product Specific Protocols
WB protocol for TLE3 antibody 11372-1-APDownload protocol
IHC protocol for TLE3 antibody 11372-1-APDownload protocol
IF protocol for TLE3 antibody 11372-1-APDownload protocol
IP protocol for TLE3 antibody 11372-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Distinct structural classes of activating FOXA1 alterations in advanced prostate cancer.

Authors - Abhijit Parolia
  • KD Validated

Cell Stem Cell

Lats1/2 Sustain Intestinal Stem Cells and Wnt Activation through TEAD-Dependent and Independent Transcription.

Authors - Qi Li

Cell Metab

TLE3 is a dual-function transcriptional coregulator of adipogenesis.

Authors - Villanueva Claudio J CJ

Cell Metab

Adipose subtype-selective recruitment of TLE3 or Prdm16 by PPARγ specifies lipid storage versus thermogenic gene programs.

Authors - Villanueva Claudio J CJ
  • KO Validated

J Clin Invest

Small molecule JQ1 promotes prostate cancer invasion via BET-independent inactivation of FOXA1.

Authors - Leiming Wang

J Exp Med

Tle corepressors are differentially partitioned to instruct CD8+ T cell lineage choice and identity.

Authors - Shaojun Xing
  • KO Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Tongbin (Verified Customer) (08-05-2020)

I did western blots using mouse heart samples. I observed a TLE3 band with correct molecular weight of about 80kD.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse heart tissue