Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11372-1-AP targets TLE3 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA, CUT&Tag applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1930 Product name: Recombinant human TLE3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC015729 Sequence: MYPQGRHPAPHQPGQPGFKFTVAESCDRIKDEFQFLQAQYHSLKVEYDKLANEKTEMQRHYVMYYEMSYGLNIEMHKQTEIAKRLNTILAQIMPFLSQEHQQQVAQAVERAKQVTMTELNAIIGQQQLQAQHLSHATHGPPVQLPPHPSGLQPPGIPPVTGSSSGLLALGALGSQAHLTVKDEKNHHELDHRERESSANNSVSPSESLRASEKHRGSADYSMEAKKRKAEEKDSLSRYDSDGDKSDDLVVDVSNEDPATPRVSPAHSPPENGLDKARSLKKDAPTSPASVASSSSTPSSKTKDLGHNDKSSTPGLKSNTPTPRNDAPTPGTSTTPGLRSMPGKPPGMDPI Predict reactive species |
| Full Name | transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila) |
| Calculated Molecular Weight | 772 aa, 83 kDa |
| Observed Molecular Weight | 83 kDa |
| GenBank Accession Number | BC015729 |
| Gene Symbol | TLE3 |
| Gene ID (NCBI) | 7090 |
| RRID | AB_2203743 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q04726 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TLE3, also named as KIAA1547 and ESG3, belongs to the WD repeat Groucho/TLE family. It is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. TLE3 is a member of the transducin-like enhancer of split (TLE) family of proteins that have been implicated in the tumorgenesis and classification of sarcomas. It is a candidate biomarker of response to taxane
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TLE3 antibody 11372-1-AP | Download protocol |
| IHC protocol for TLE3 antibody 11372-1-AP | Download protocol |
| IP protocol for TLE3 antibody 11372-1-AP | Download protocol |
| WB protocol for TLE3 antibody 11372-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TLE3 antibody (Cat# 11372-1-AP) has 26 citations spanning journals including Nature, Cell Metabolism, Nature Immunology, Cell Stem Cell, Nature Cell Biology, Cancer Research, JCI, JEM, EMBO Journal, Genes & Development, Cell Reports, FASEB Journal, JBC, PLoS Pathogens, and Oncotarget. Research fields cover transcriptional regulation, cancer (prostate, breast, thyroid, melanoma, myeloma), T-cell differentiation, adipogenesis/metabolism, intestinal stem cells, neurobiology, and inflammation.
| Species | Application | Title |
|---|---|---|
Nature Distinct structural classes of activating FOXA1 alterations in advanced prostate cancer.
| ||
Cell Metab Adipose subtype-selective recruitment of TLE3 or Prdm16 by PPARγ specifies lipid storage versus thermogenic gene programs.
| ||
Nat Immunol The transcriptional cofactor Tle3 reciprocally controls effector and central memory CD8+ T cell fates | ||
Nat Immunol Phosphorylation of Runx protein controls helper CD4(+) T cell versus cytotoxic CD8(+) T cell lineage choice. | ||
Cell Stem Cell Lats1/2 Sustain Intestinal Stem Cells and Wnt Activation through TEAD-Dependent and Independent Transcription. |
Reviews
What customers say
The single review for this product reports successful Western Blot results using mouse heart tissue at a 1:1000 dilution. The user observed a TLE3 band at the expected molecular weight of approximately 80 kD, and rated the product 5 stars. Overall, the feedback is positive, confirming the antibody's specificity and performance in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tongbin (Verified Customer) (08-05-2020) | I did western blots using mouse heart samples. I observed a TLE3 band with correct molecular weight of about 80kD.
|
