TLE3 Polyclonal antibody

• Featured Product
• KD/KO Validated

TLE3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

17

Reviews (1)

Cat no : 11372-1-AP

Synonyms

ESG, ESG3, GRG3, HsT18976, KIAA1547, TLE3

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Validation Data Gallery

• 

  WB analysis using 11372-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11372-1-AP (TLE3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11372-1-AP

  WB result of TLE3 antibody (11372-1-AP, 1:500) with si-Control and si-TLE3 transfected HEK293 cells.

• 

  WB analysis of HeLa using 11372-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11372-1-AP (TLE3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 11372-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 11372-1-AP (TLE3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11372-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11372-1-AP (TLE3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11372-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11372-1-AP (TLE3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11372-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11372-1-AP (TLE3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11372-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11372-1-AP (TLE3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11372-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11372-1-AP (TLE3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 11372-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11372-1-AP (TLE3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11372-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11372-1-AP (TLE3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11372-1-AP

  IP Result of anti-TLE3 (IP:11372-1-AP, 4ug; Detection:11372-1-AP 1:300) with HeLa cells lysate 1600ug.

• 

  IHC staining of human breast cancer using 11372-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 11372-1-AP (TLE3 Antibody) at dilution of 1:50.

• 

  IHC staining of human lung cancer using 11372-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 11372-1-AP (TLE3 Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 11372-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 11372-1-AP (TLE3 Antibody) at dilution of 1:50.

• 

  IHC staining of human lung cancer using 11372-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 11372-1-AP (TLE3 Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 11372-1-AP

  Immunofluorescent analysis of HepG2 cells using 11372-1-AP (TLE3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, human lung tissue, A431 cells, HEK-293 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 6 publications below
• WB: See 14 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• CoIP: See 2 publications below
• ChIP: See 7 publications below

Product Information

11372-1-AP targets TLE3 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TLE3 fusion protein Ag1930
• Full Name: transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
• Calculated molecular weight: 772 aa, 83 kDa
• Observed molecular weight: 83 kDa
• GenBank accession number: BC015729
• Gene symbol: TLE3
• Gene ID (NCBI): 7090
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TLE3, also named as KIAA1547 and ESG3, belongs to the WD repeat Groucho/TLE family. It is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. TLE3 is a member of the transducin-like enhancer of split (TLE) family of proteins that have been implicated in the tumorgenesis and classification of sarcomas. It is a candidate biomarker of response to taxane

Publications

Species	Application	Title
human	WB,CoIP,ChIP	Nature; Distinct structural classes of activating FOXA1 alterations in advanced prostate cancer.; Authors - Abhijit Parolia ; KD Validated
mouse	WB	Cell Stem Cell; Lats1/2 Sustain Intestinal Stem Cells and Wnt Activation through TEAD-Dependent and Independent Transcription.; Authors - Qi Li
mouse	WB,ChIP	Cell Metab; TLE3 is a dual-function transcriptional coregulator of adipogenesis.; Authors - Villanueva Claudio J CJ
mouse	WB	Cell Metab; Adipose subtype-selective recruitment of TLE3 or Prdm16 by PPARγ specifies lipid storage versus thermogenic gene programs.; Authors - Villanueva Claudio J CJ ; KO Validated
human	WB,chIP	J Clin Invest; Small molecule JQ1 promotes prostate cancer invasion via BET-independent inactivation of FOXA1.; Authors - Leiming Wang
mouse	chIP	J Exp Med; Tle corepressors are differentially partitioned to instruct CD8+ T cell lineage choice and identity.; Authors - Shaojun Xing ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tongbin (Verified Customer) (08-05-2020)

I did western blots using mouse heart samples. I observed a TLE3 band with correct molecular weight of about 80kD.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart tissue

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