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Tested Applications
| Positive WB detected in | MDA-MB-231 cells, OVCAR-3 cells, U266 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32730-1-AP targets TMCC3 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38430 Product name: Recombinant human TMCC3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 120-250 aa of NM_001301036.1 Sequence: QKSAHSIAQLQKKLEQYHRKLREIEQNGASRSSKDISKDHLKDIHRSLKDAHVKSRTAPHCMESSKSGMPGVSLTPPVFVFNKSREFANLIRNKFGSADNIAHLKNSLEEFRPEASARAYGGSATIVNKPK Predict reactive species |
| Full Name | transmembrane and coiled-coil domain family 3 |
| Calculated Molecular Weight | 54kDa，477aa |
| Observed Molecular Weight | 54 kDa |
| GenBank Accession Number | NM_001301036.1 |
| Gene Symbol | TMCC3 |
| Gene ID (NCBI) | 57458 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9ULS5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TMCC3 is a member of the transmembrane and coiled-coil domain (TMCC) family of proteins, which are integral membrane proteins localized in the endoplasmic reticulum (ER). The TMCC family consists of three members: TMCC1, TMCC2, and TMCC3, which are conserved from nematodes to humans. (PMID: 33742122)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TMCC3 antibody 32730-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
