TMEM106B (150-274aa) Polyclonal antibody

• Featured Product
• KD/KO Validated

TMEM106B (150-274aa) Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

9

Reviews (1)

Cat no : 20995-1-AP

Synonyms

TMEM106B, Transmembrane protein 106B

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Validation Data Gallery

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  WB analysis using 20995-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20995-1-AP (TMEM106B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20995-1-AP

  WB result of TMEM106B antibody (20995-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM106B transfected HeLa cells.

• 

  IHC staining of rat brain using 20995-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20995-1-AP (TMEM106B (150-274aa) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 20995-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20995-1-AP (TMEM106B (150-274aa) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, HepG2 cells
• Positive IHC detected in: rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IF: See 3 publications below

Product Information

20995-1-AP targets TMEM106B (150-274aa) in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMEM106B (150-274aa) fusion protein Ag14264
• Full Name: transmembrane protein 106B
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 31-35 kDa, 40-55 kDa
• GenBank Accession Number: BC033901
• Gene Symbol: TMEM106B
• Gene ID (NCBI): 54664
• RRID: AB_10694293
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM106B is a genetic risk factor for frontotemporal lobar degeneration with TDP-43 inclusions (FTLD-TDP). Amyotrophic lateral sclerosis (ALS), like FTLD-TDP, is characterized by pathological TDP-43 inclusions. TMEM106B expression in the brain may be linked to mechanisms of disease in FTLD-TDP and risk alleles confer genetic susceptibility by increasing gene expression. TMEM106B can be showed as 31-55 kDa and 70-90 kDa (Glycosylated or Dimer) form in western blot test. (PMID: 27543298, 22895706, PMID: 23136129).

Publications

Species	Application	Title
human		Cell; Homotypic fibrillization of TMEM106B across diverse neurodegenerative diseases.; Authors - Andrew Chang
human	WB	J Neuroinflammation; Progranulin haploinsufficiency mediates cytoplasmic TDP-43 aggregation with lysosomal abnormalities in human microglia; Authors - Wonjae Sung
human	WB	J Neurosci; TMEM106B, the risk gene for frontotemporal dementia, is regulated by the microRNA-132/212 cluster and affects progranulin pathways.; Authors - Chen-Plotkin Alice S AS
human	WB	Alzheimers Res Ther; TMEM106B expression is reduced in Alzheimer's disease brains.; Authors - Jun-Ichi Satoh
mouse	IF	Mol Brain; TMEM106B, a frontotemporal lobar dementia (FTLD) modifier, associates with FTD-3-linked CHMP2B, a complex of ESCRT-III.; Authors - Mi-Hee Jun
human	WB	PLoS One; Construction of ceRNA network to identify the lncRNA and mRNA related to non-small cell lung cancer.; Authors - Kui Xiao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Carolyn (Verified Customer) (02-18-2022)

good signal, low background

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human brain tissue extract