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  • KD/KO Validated

TMEM173/STING Polyclonal antibody

TMEM173/STING Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IP, IHC, IF, FC, CoIP, Blocking assay, ELISA

Conjugate

Unconjugated

Cat no : 19851-1-AP

Synonyms

hMITA, hSTING, Mediator of IRF3 activation, MITA, MPYS, NET23, STING, STING1, TMEM173, TMEM173/STING, transmembrane protein 173



Tested Applications

Positive WB detected inHEK-293 cells, HL-60 cells, THP-1 cells, mouse thymus tissue, mouse spleen tissue, Raji cells, human heart tissue, HT-29 cells, C2C12 cells, HepG2 cells
Positive IP detected inmouse spleen tissue
Positive IHC detected inhuman tonsillitis tissue, human liver cancer tissue, rat spleen tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHT-29 cells, human tonsillitis tissue, HepG2 cells
Positive FC detected inMCF-7 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 19851-1-AP is TMEM173/STING Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, Marc-145 cells, monkey, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM173/STING fusion protein Ag13921
Full Name transmembrane protein 173
Calculated molecular weight 379 aa, 42 kDa
Observed molecular weight 35-40 kDa, 80 kDa
GenBank accession numberBC047779
Gene symbol TMEM173
Gene ID (NCBI) 340061
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines. STING is a 379 amino acid protein with a calculated molecular weight of 42 kDa. It has been observed at 35-40 kDa (PMID: 27324217; 29632140; 30918080), and 70-80 kDa corresponding to the expected size of a STING dimer (PMID: 25790474; 29491158).

Protocols

Product Specific Protocols
WB protocol for TMEM173/STING antibody 19851-1-APDownload protocol
IHC protocol for TMEM173/STING antibody 19851-1-APDownload protocol
IF protocol for TMEM173/STING antibody 19851-1-APDownload protocol
IP protocol for TMEM173/STING antibody 19851-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cell Death Differ

STING signaling remodels the tumor microenvironment by antagonizing myeloid-derived suppressor cell expansion.

Authors - Chuan-Xia Zhang
humanWB,IF

Nature

Autophagy induction via STING trafficking is a primordial function of the cGAS pathway.

Authors - Xiang Gui
  • KO Validated
mouseWB

Int J Mol Sci

cGAS/STING/TBK1/IRF3 Signaling Pathway Activates BMDCs Maturation Following Mycobacterium bovis Infection.

Authors - Qiang Li

J Virol

African swine fever virus Armenia/07 virulent strain controls IFN-β production through cGAS-STING pathway.

Authors - Raquel García-Belmonte
mouseWB,IF

Front Immunol

DNA Sensor IFI204 Contributes to Host Defense Against Staphylococcus aureus Infection in Mice.

Authors - Wei Chen
mouseWB

PLoS Pathog

USP49 negatively regulates cellular antiviral responses via deconjugating K63-linked ubiquitination of MITA.

Authors - Liya Ye

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Will (Verified Customer) (04-25-2019)

good antibody, will order again

  • Applications: Western Blot,