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  • KD/KO Validated

TMEM173/STING Polyclonal antibody

TMEM173/STING Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 19851-1-AP

Synonyms

hMITA, hSTING, Mediator of IRF3 activation, MITA, MPYS, NET23, STING, TMEM173, transmembrane protein 173



Tested Applications

Positive WB detected inHEK-293 cells, HL-60 cells, HT-29 cells, HepG2 cells, human heart tissue, mouse spleen tissue, Raji cells, rat heart tissue, mouse thymus
Positive IP detected inmouse spleen tissue
Positive IHC detected inhuman spleen tissue, human tonsillitis tissue, rat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inMCF-7 cells
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 19851-1-AP is TMEM173/STING Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM173/STING fusion protein Ag13921
Full Name transmembrane protein 173
Calculated molecular weight 379 aa, 42 kDa
Observed molecular weight 35-40 kDa, 80 kDa
GenBank accession numberBC047779
Gene symbol TMEM173
Gene ID (NCBI) 340061
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TMEM173, also named as ERIS, MITA, STING and MPYS, is a facilitator of innate immune signaling that promotes the production of type I IFN (IFN-alpha and IFN-beta). TMEM173 mediates death signaling via activation of the extracellular signal-regulated kinase (ERK) pathway. There’re monomer (35-40 kDa)(PMID: 27324217; 29632140;30918080) and dimer (80 kDa) form of TMEM173 in western blot test.

Protocols

Product Specific Protocols
WB protocol for TMEM173/STING antibody 19851-1-APDownload protocol
IHC protocol for TMEM173/STING antibody 19851-1-APDownload protocol
IF protocol for TMEM173/STING antibody 19851-1-APDownload protocol
IP protocol for TMEM173/STING antibody 19851-1-APDownload protocol
FC protocol for TMEM173/STING antibody 19851-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cell Death Differ

STING signaling remodels the tumor microenvironment by antagonizing myeloid-derived suppressor cell expansion.

Authors - Chuan-Xia Zhang
humanWB,IF

Nature

Autophagy induction via STING trafficking is a primordial function of the cGAS pathway.

Authors - Xiang Gui
  • KO Validated
mouseWB

Int J Mol Sci

cGAS/STING/TBK1/IRF3 Signaling Pathway Activates BMDCs Maturation Following Mycobacterium bovis Infection.

Authors - Qiang Li

J Virol

African swine fever virus Armenia/07 virulent strain controls IFN-β production through cGAS-STING pathway.

Authors - Raquel García-Belmonte
mouseWB,IF

Front Immunol

DNA Sensor IFI204 Contributes to Host Defense Against Staphylococcus aureus Infection in Mice.

Authors - Wei Chen
mouseWB

PLoS Pathog

USP49 negatively regulates cellular antiviral responses via deconjugating K63-linked ubiquitination of MITA.

Authors - Liya Ye

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Will (Verified Customer) (04-25-2019)

good antibody, will order again

  • Applications: Western Blot,