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TMEM173/STING Polyclonal antibody
TMEM173/STING Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 19851-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, HL-60 cells, THP-1 cells, mouse thymus tissue, mouse spleen tissue, Raji cells, human heart tissue, HT-29 cells, C2C12 cells, HepG2 cells|
|Positive IP detected in||mouse spleen tissue|
|Positive IHC detected in||human tonsillitis tissue, human liver cancer tissue, rat spleen tissue, human spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HT-29 cells, human tonsillitis tissue, HepG2 cells|
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:2000-1:8000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 19851-1-AP is TMEM173/STING Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, Marc-145 cells, monkey, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TMEM173/STING fusion protein Ag13921|
|Full Name||transmembrane protein 173|
|Calculated molecular weight||379 aa, 42 kDa|
|Observed molecular weight||35-40 kDa, 80 kDa|
|GenBank accession number||BC047779|
|Gene ID (NCBI)||340061|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines. STING is a 379 amino acid protein with a calculated molecular weight of 42 kDa. It has been observed at 35-40 kDa (PMID: 27324217; 29632140; 30918080), and 70-80 kDa corresponding to the expected size of a STING dimer (PMID: 25790474; 29491158).
|Product Specific Protocols|
|WB protocol for TMEM173/STING antibody 19851-1-AP||Download protocol|
|IHC protocol for TMEM173/STING antibody 19851-1-AP||Download protocol|
|IF protocol for TMEM173/STING antibody 19851-1-AP||Download protocol|
|IP protocol for TMEM173/STING antibody 19851-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Cell Death Differ
STING signaling remodels the tumor microenvironment by antagonizing myeloid-derived suppressor cell expansion.
Autophagy induction via STING trafficking is a primordial function of the cGAS pathway.
Int J Mol Sci
cGAS/STING/TBK1/IRF3 Signaling Pathway Activates BMDCs Maturation Following Mycobacterium bovis Infection.
African swine fever virus Armenia/07 virulent strain controls IFN-β production through cGAS-STING pathway.
DNA Sensor IFI204 Contributes to Host Defense Against Staphylococcus aureus Infection in Mice.
USP49 negatively regulates cellular antiviral responses via deconjugating K63-linked ubiquitination of MITA.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Will (Verified Customer) (04-25-2019)
good antibody, will order again