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  • KD/KO Validated

TMEM173/STING Polyclonal antibody

TMEM173/STING Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, FC, CoIP, Blocking assay, ELISA

Conjugate

Unconjugated

Cat no : 19851-1-AP

Synonyms

hMITA, hSTING, Mediator of IRF3 activation, MITA, MPYS, NET23, STING, STING1, TMEM173, TMEM173/STING, transmembrane protein 173



Tested Applications

Positive WB detected inHEK-293 cells, mouse kidney tissue, HepG2 cells, Raji cells, mouse spleen tissue, THP-1 cells, HL-60 cells, mouse testis tissue, HT-29 cells, mouse thymus tissue, C2C12 cells
Positive IP detected inmouse spleen tissue
Positive IHC detected inhuman tonsillitis tissue, rat thymus tissue, mouse thymus tissue, human spleen tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHT-29 cells, human tonsillitis tissue
Positive FC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

19851-1-AP targets TMEM173/STING in WB, IP, IHC, IF, FC, CoIP, Blocking assay, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM173/STING fusion protein Ag13921
Full Name transmembrane protein 173
Calculated molecular weight 379 aa, 42 kDa
Observed molecular weight 35-40 kDa, 80 kDa
GenBank accession numberBC047779
Gene symbol TMEM173
Gene ID (NCBI) 340061
RRIDAB_10665370
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines. STING is a 379 amino acid protein with a calculated molecular weight of 42 kDa. It has been observed at 35-40 kDa (PMID: 27324217; 29632140; 30918080), and 70-80 kDa corresponding to the expected size of a STING dimer (PMID: 25790474; 29491158).

Protocols

Product Specific Protocols
WB protocol for TMEM173/STING antibody 19851-1-APDownload protocol
IHC protocol for TMEM173/STING antibody 19851-1-APDownload protocol
IF protocol for TMEM173/STING antibody 19851-1-APDownload protocol
IP protocol for TMEM173/STING antibody 19851-1-APDownload protocol
FC protocol for TMEM173/STING antibody 19851-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nature

Tonic prime-boost of STING signalling mediates Niemann-Pick disease type C.

Authors - Ting-Ting Chu
  • KO Validated
mouseWB

Nature

eccDNAs are apoptotic products with high innate immunostimulatory activity

Authors - Yuangao Wang
  • KO Validated
humanWB

Science

The heme-regulated inhibitor is a cytosolic sensor of protein misfolding that controls innate immune signaling.

Authors - Mena Abdel-Nour
humanWB,IF

Signal Transduct Target Ther

TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion

Authors - Wenwen Zheng
  • KD Validated
humanWB

Cancer Cell

Mutant p53 suppresses innate immune signaling to promote tumorigenesis.

Authors - Monisankar Ghosh
  • KD Validated
mouseIF

Nat Immunol

Homeostatic regulation of STING protein at the resting state by stabilizer TOLLIP.

Authors - Vladislav Pokatayev

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Will (Verified Customer) (04-25-2019)

good antibody, will order again

  • Applications: Western Blot,