TMEM192 Polyclonal antibody, PBS Only

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  WB analysis using 28263-1-AP (same clone as 28263-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28263-1-AP (TMEM192 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28263-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 28263-1-AP (same clone as 28263-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28263-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 28263-1-AP (same clone as 28263-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28263-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 28263-1-AP (same clone as 28263-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28263-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 28263-1-AP (same clone as 28263-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28263-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 28263-1-AP (same clone as 28263-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28263-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 28263-1-AP (same clone as 28263-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28263-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 28263-1-AP (same clone as 28263-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28263-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 28263-1-AP (same clone as 28263-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28263-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 28263-1-AP (same clone as 28263-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28263-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 28263-1-AP (same clone as 28263-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28263-1-PBS in a different storage buffer formulation.

TMEM192 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 28263-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

28263-1-PBS targets TMEM192 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27576

  Product name: Recombinant human TMEM192 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 193-271 aa of BC036301

  Sequence: KIRRFNKAKPEPDILEEEKIYAYPSNITSETGFRTISSLEEIVEKQGDTIEYLKRHNALLSKRLLALTSSDLGCQPSRT

• Full Name: transmembrane protein 192
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC036301
• Gene Symbol: TMEM192
• Gene ID (NCBI): 201931
• RRID: AB_2881099
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IY95
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Transmembrane protein 192 (TMEM192) has been previously identified in proteomic analyses of lysosomal membranes.TMEM192 shows a wide expression pattern in mouse tissues. Interestingly, TMEM192 was found to be highly expressed in tumor cell lines, while it was not expressed or detected at low levels in normal cell lines(PMID: 22736246). TMEM192 can be detected as 30 kDa.