Tested Applications
| Positive WB detected in | BxPC-3 cells, L02 cells, Hela cells, HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human cervical cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19728-1-AP targets TMF1-Specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | TATA element modulatory factor 1 |
| Calculated Molecular Weight | 123 kDa |
| Observed Molecular Weight | 150-160 kDa |
| GenBank Accession Number | NM_007114 |
| Gene Symbol | TMF1 |
| Gene ID (NCBI) | 7110 |
| RRID | AB_10667009 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P82094 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TMF1, also named as ARA160, is a potential coactivator of the androgen receptor. TMF1 mediates STAT3 degradation. TMF1 plays critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER. TMF1 binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP). The antibody is specific to TMF1. TMF1 can be detected as 150-160 kDa after posttranslational modification.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TMF1-Specific antibody 19728-1-AP | Download protocol |
| IHC protocol for TMF1-Specific antibody 19728-1-AP | Download protocol |
| IP protocol for TMF1-Specific antibody 19728-1-AP | Download protocol |
| WB protocol for TMF1-Specific antibody 19728-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TMF1-Specific Polyclonal antibody (Cat# 19728-1-AP) has 2 total citations. These appear in Molecular Cell (IF 16) and Journal of Biological Chemistry (IF 4.1). The cited research fields include unconventional secretion and extracellular proteome remodeling under stress (mTORC1-GRASP55 signaling), as well as proteomic identification of molecular links between the coatomer protein complex and androgen receptor-dependent transcription.
| Species | Application | Title |
|---|---|---|
Mol Cell An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress. | ||
J Biol Chem Discovery Proteomics Identifies a Molecular Link between the Coatomer Protein Complex I and Androgen Receptor-dependent Transcription. |
