TMOD4 Polyclonal antibody

TMOD4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 11753-1-AP

Synonyms

SK TMOD, Skeletal muscle tropomodulin, TMOD4, Tropomodulin 4, tropomodulin 4 (muscle)

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 11753-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11753-1-AP (TMOD4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11753-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11753-1-AP (TMOD4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11753-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11753-1-AP (TMOD4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 11753-1-AP

  IP result of anti-TMOD4 (IP:11753-1-AP, 4ug; Detection:11753-1-AP 1:600) with mouse skeletal muscle tissue lysate 3600ug.

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  IHC staining of human gliomas using 11753-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11753-1-AP (TMOD4 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, HeLa cells, human brain tissue
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

11753-1-AP targets TMOD4 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat, Branchiostoma belcheri, zebrafish, Xenopus
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMOD4 fusion protein Ag2349
• Full Name: tropomodulin 4 (muscle)
• Calculated Molecular Weight: 345 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC017810
• Gene Symbol: TMOD4
• Gene ID (NCBI): 29765
• RRID: AB_2205433
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMOD4 is a member of tropomodulin (Tmod) family proteins which are capping proteins binding to the pointed end of actin filaments and preventing both elongation and depolymerization. Four mammalian Tmod genes have been identified to date. Tomd1 is expressed in terminally-differentiated post-mitotic cells, like heart, brain, lens, vasculature, and erythrocytes; Tmod2 expressed in neurons; Tmod3 expressed ubiquitously; Tmod4 expressed only in skeletal muscles. This antibody was raised against the full-length human TMOD4 and may cross-react with other Tmods.

Publications

Species	Application	Title
mouse	WB	Elife; Downsizing the molecular spring of the giant protein titin reveals that skeletal muscle titin determines passive stiffness and drives longitudinal hypertrophy.; Authors - Ambjorn Brynnel
Xenopus	WB, IF	J Cell Sci; Leiomodin 3 and Tropomodulin 4 have overlapping functions during skeletal myofibrillogenesis.; Authors - Chinedu U Nworu
zebrafish	IHC	Dis Model Mech; Neb: a zebrafish model of nemaline myopathy due to nebulin mutation.; Authors - Telfer William R WR
zebrafish	IF	Dis Model Mech; Loss of tropomodulin4 in the zebrafish mutant träge causes cytoplasmic rod formation and muscle weakness reminiscent of nemaline myopathy.; Authors - Joachim Berger
rat	WB	J Proteome Res; Proteomic and Phosphoproteomic Analysis of Right Ventricular Hypertrophy in the Pulmonary Hypertension Rat Model; Authors - Ang Luo
rat	IF	Front Physiol; Titin isoform size is not correlated with thin filament length in rat skeletal muscle.; Authors - Marion L Greaser