• Featured Product
  • KD/KO Validated

TMPO/LAP2 Polyclonal antibody

TMPO/LAP2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (2)

Applications

WB, IP, IHC, IF

Conjugate

Unconjugated

Cat no : 14651-1-AP

Synonyms

CMD1T, LAP2, LEMD4, PRO0868, Splenin Thymopentin, thymopoietin, Thymopoietin isoform alpha, TMPO, TMPO/LAP2, TP, TP alpha, TP5, TPRP isoform alpha



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14651-1-AP targets TMPO/LAP2 in WB, IP, IHC, IF applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivitybovine, human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMPO/LAP2 fusion protein Ag6247
Full Name thymopoietin
Calculated molecular weight 51 kDa
Observed molecular weight 75 kDa, 51 kDa and 39 kDa
GenBank accession numberBC053675
Gene symbol TMPO
Gene ID (NCBI) 7112
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TMPO resides in the nucleus and may play a role in the assembly of the nuclear lamina, and thus help maintain the structural organization of the nuclear envelope. It may function as a receptor for the attachment of lamin filaments to the inner nuclear membrane. TMPO gene encodes 3 thymopoietins: alpha (75 kD), beta (51 kD), and gamma (39 kD).

Protocols

Product Specific Protocols
WB protocol for TMPO/LAP2 antibody 14651-1-APDownload protocol
IHC protocol for TMPO/LAP2 antibody 14651-1-APDownload protocol
IP protocol for TMPO/LAP2 antibody 14651-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Theranostics

Progerin accumulation in nucleus pulposus cells impairs mitochondrial function and induces intervertebral disc degeneration and therapeutic effects of sulforaphane.

Authors - Xiaolong Xu
humanWB

World J Surg Oncol

Depletion of thymopoietin inhibits proliferation and induces cell cycle arrest/apoptosis in glioblastoma cells.

Authors - Lin Zhang
  • KD Validated
bovineWB

J Dairy Sci

Anti-inflammation of epicatechin mediated by TMEM35A and TMPO in bovine mammary epithelial cell line cells and mouse mammary gland.

Authors - Xiao Ma
  • KO Validated
humanWB

Sci Rep

MacroH2A1 associates with nuclear lamina and maintains chromatin architecture in mouse liver cells.

Authors - Yuhua Fu
humanIF

J Cell Biol

A toolbox of anti-mouse and anti-rabbit IgG secondary nanobodies.

Authors - Tino Pleiner
mouse

Nat Commun

The missing linker between SUN5 and PMFBP1 in sperm head-tail coupling apparatus.

Authors - Ying Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Lauren (Verified Customer) (02-20-2020)

Tested fixes include -20'C Methanol for 10 mins, and both 2% and 4% Formaldehyde for 20 mins. We saw a good signal in all three fixes.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: mIMCD3
TMPO/LAP2 Antibody Immunofluorescence, validation (1:100 dilution) in mIMCD3 (Cat no:14651-1-AP)