|Positive WB detected in||HEK-293 cells, HeLa cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human breast cancer tissue, mouse lung tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
14651-1-AP targets TMPO/LAP2 in WB, IP, IHC, IF applications and shows reactivity with human, rat samples.
|Tested Reactivity||human, rat|
|Cited Reactivity||bovine, human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TMPO/LAP2 fusion protein Ag6247|
|Calculated molecular weight||51 kDa|
|Observed molecular weight||75 kDa, 51 kDa and 39 kDa|
|GenBank accession number||BC053675|
|Gene ID (NCBI)||7112|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
TMPO resides in the nucleus and may play a role in the assembly of the nuclear lamina, and thus help maintain the structural organization of the nuclear envelope. It may function as a receptor for the attachment of lamin filaments to the inner nuclear membrane. TMPO gene encodes 3 thymopoietins: alpha (75 kD), beta (51 kD), and gamma (39 kD).
Progerin accumulation in nucleus pulposus cells impairs mitochondrial function and induces intervertebral disc degeneration and therapeutic effects of sulforaphane.
World J Surg Oncol
Depletion of thymopoietin inhibits proliferation and induces cell cycle arrest/apoptosis in glioblastoma cells.
J Dairy Sci
Anti-inflammation of epicatechin mediated by TMEM35A and TMPO in bovine mammary epithelial cell line cells and mouse mammary gland.
MacroH2A1 associates with nuclear lamina and maintains chromatin architecture in mouse liver cells.
J Cell Biol
A toolbox of anti-mouse and anti-rabbit IgG secondary nanobodies.
The missing linker between SUN5 and PMFBP1 in sperm head-tail coupling apparatus.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Lauren (Verified Customer) (02-20-2020)
Tested fixes include -20'C Methanol for 10 mins, and both 2% and 4% Formaldehyde for 20 mins. We saw a good signal in all three fixes.